Western Blot (WB)
(Western Blot (WB) analysis of RAB5A polyclonal antibody in extracts from Hela, PC12 cells and mouse brain tissue.)
Rabbit RAB5A Polyclonal Antibody | anti-RAB5A antibody
RAB5A Polyclonal Antibody
Gene Names
RAB5A; RAB5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
RAB5A; Polyclonal Antibody; RAB5A Polyclonal Antibody; Ras-related protein Rab-5A; RAB5; anti-RAB5A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RAB5A polyclonal antibody detects endogenous levels of RAB5A protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
215
Applicable Applications for anti-RAB5A antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant full length Human RAB5A.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of RAB5A polyclonal antibody in extracts from Hela, PC12 cells and mouse brain tissue.)
Western Blot (WB)
(Western Blot (WB) analysis of RAB5A polyclonal antibody in extracts from Hela, PC12 cells and mouse brain tissue.)
Immunofluorescence (IF)
(Immunofluorescence analysis of Hela cells, using RAB5A polyclonal antibody)
Immunofluorescence (IF)
(Immunofluorescence analysis of Hela cells, using RAB5A polyclonal antibody)
Related Product Information for anti-RAB5A antibody
The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, RAP, Ral/Rec and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves, at each stage, the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 24kDa
NCBI Official Full Name
ras-related protein Rab-5A isoform 1
NCBI Official Synonym Full Names
RAB5A, member RAS oncogene family
NCBI Official Symbol
RAB5A
NCBI Official Synonym Symbols
RAB5
NCBI Protein Information
ras-related protein Rab-5A
UniProt Protein Name
Ras-related protein Rab-5A
Protein Family
UniProt Gene Name
RAB5A
UniProt Synonym Gene Names
RAB5
UniProt Entry Name
RAB5A_HUMAN
Research Articles on RAB5A
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Product Notes
The RAB5A rab5a (Catalog #AAA3007761) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB5A Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB5A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the RAB5A rab5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB5A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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