Rabbit Rab 5C Polyclonal Antibody | anti-Rab5C antibody

Rab 5C (A153) Polyclonal Antibody

Cat. Num. AAA3004450

• Gene Names: RAB5C; RABL; L1880; RAB5L; RAB5CL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Rab 5C; Polyclonal Antibody; Rab 5C (A153) Polyclonal Antibody; RAB; member of RAS oncogene family like; RAB5C; member of RAS oncogene family; member RAS oncogene family; RAB5CL; RAB5L; RABL; Ras-related protein Rab-5C; anti-Rab5C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Rab 5C (A153) polyclonal antibody detects endogenous levels of Rab 5C protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 249

• Applicable Applications for anti-Rab5C antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 130-180 of Human Rab 5C.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Rab 5C (A153) pAb at 1:1000 dilutionLane1:A375 whole cell lysate (40ug) Lane2:Hela whole cell lysate (30ug) Lane3:The Lung tissue lysate of Rat (40ug) Lane4:The Lung tissue lysate of Mouse (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Rab 5C (A153) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.)

Related Product Information for anti-Rab5C antibody

The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles.

NCBI and Uniprot Product Information

• NCBI GI #: 354721184
• NCBI GeneID: 5878
• NCBI Accession #: NP_001238968.1
• NCBI GenBank Nucleotide #: NP_001238968.1
• UniProt Accession #: P51148
• Molecular Weight: ~ 24kDa
• NCBI Official Full Name: ras-related protein Rab-5C isoform b
• NCBI Official Synonym Full Names: RAB5C, member RAS oncogene family
• NCBI Official Symbol: RAB5C
• NCBI Official Synonym Symbols: RABL; L1880; RAB5L; RAB5CL
• NCBI Protein Information: ras-related protein Rab-5C
• UniProt Protein Name: Ras-related protein Rab-5C
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB5C
• UniProt Synonym Gene Names: RABL
• UniProt Entry Name: RAB5C_HUMAN

NCBI Description

Members of the Rab protein family are small GTPases of the Ras superfamily that are thought to ensure fidelity in the process of docking and/or fusion of vesicles with their correct acceptor compartment (Han et al., 1996 [PubMed 8646882]).[supplied by OMIM, Nov 2010]

Uniprot Description

RAB5C: Protein transport. Probably involved in vesicular traffic. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; Motility/polarity/chemotaxis; G protein; G protein, monomeric

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: intracellular membrane-bound organelle; lysosomal membrane; endocytic vesicle; early endosome membrane; early endosome; plasma membrane; melanosome; lipid particle

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; metabolic process; regulation of endocytosis; plasma membrane to endosome transport; Rab protein signal transduction