Rabbit anti-Human MRPS16 Polyclonal Antibody | anti-MRPS16 antibody

MRPS16 Antibody

Cat. Num. AAA7044642

• Gene Names: MRPS16; COXPD2; RPMS16; CGI-132; MRP-S16
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: MRPS16; Polyclonal Antibody; MRPS16 Antibody; 28S ribosomal protein S16; mitochondrial; MRP-S16; S16mt; RPMS16; CGI-132; anti-MRPS16 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 137

• Applicable Applications for anti-MRPS16 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human 28S ribosomal protein S16, mitochondrial protein (1-137AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: MRPS16 antibody at 1.12 ug/mlLane 1: 293T whole cell lysateLane 2: Jurkat whole cell lysateLane 3: Hela whole cell lysateLane 4: MCF7 whole cell lysateLane 5: Raji whole cell lysateLane 6: HepG-2 whole cell lysateSecondaryGoat polyclonal to rabbit IgG at 1/10000 dilutionPredicted band size: 16,14 kDaObserved band size: 15,55 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human heart tissue using MBS7044642 at dilution of 1:100)

Related Product Information for anti-MRPS16 antibody

cytoplasm, mitochondrial inner membrane, mitochondrial small ribosomal subunit, mitochondrion, structural constituent of ribosome, mitochondrial translation, mitochondrial translational elongation, mitochondrial translational termination, translation

NCBI and Uniprot Product Information

• NCBI GI #: 7705626
• NCBI GeneID: 51021
• NCBI Accession #: NP_057149.1
• NCBI GenBank Nucleotide #: NM_016065.3
• UniProt Accession #: Q9Y3D3; Q96Q60; B4E032
• Molecular Weight: 13,829 Da
• NCBI Official Full Name: 28S ribosomal protein S16, mitochondrial
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein S16
• NCBI Official Symbol: MRPS16
• NCBI Official Synonym Symbols: COXPD2; RPMS16; CGI-132; MRP-S16
• NCBI Protein Information: 28S ribosomal protein S16, mitochondrial
• UniProt Protein Name: 28S ribosomal protein S16, mitochondrial
• Protein Family: 28S ribosomal protein
• UniProt Gene Name: MRPS16
• UniProt Synonym Gene Names: RPMS16; MRP-S16; S16mt
• UniProt Entry Name: RT16_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S16P family. The encoded protein is one of the most highly conserved ribosomal proteins between mammalian and yeast mitochondria. Three pseudogenes (located at 8q21.3, 20q13.32, 22q12-q13.1) for this gene have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPS16: Defects in MRPS16 are the cause of combined oxidative phosphorylation deficiency type 2 (COXPD2). Defects in the mitochondrial oxidative phosphorylation system result in devastating, mainly multisystem, diseases. COXPD2 symptoms include fatal neonatal metabolic acidosis with agenesis of the corpus callosum. Belongs to the ribosomal protein S16P family.

Protein type: Ribosomal; Translation; Mitochondrial

Chromosomal Location of Human Ortholog: 10q22.1

Cellular Component: cytoplasm; mitochondrial inner membrane; mitochondrial small ribosomal subunit; mitochondrion

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mitochondrial translation

Disease: Combined Oxidative Phosphorylation Deficiency 2