Immunohistochemistry (IHC)
(MBS9602219 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit RAB3IP Polyclonal Antibody | anti-RAB3IP antibody
RAB3IP Antibody
Gene Names
RAB3IP; RABIN3; RABIN8
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RAB3IP; Polyclonal Antibody; RAB3IP Antibody; Gtpat12; Rab-3A-interacting protein; RAB3A interacting protein; RAB3A interacting protein (rabin3); RAB3A-interacting protein; RAB3I_HUMAN; Rab3ip; Rabin 3; Rabin-3; RABIN3; rat; homolog of; Rabin8; SSX2 interacting protein; SSX2-interacting protein; anti-RAB3IP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
RAB3IP antibody detects endogenous levels of total RAB3IP
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
254
Applicable Applications for anti-RAB3IP antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human RAB3IP
Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, Cytoskeleton. Cell Projection, Lamellipodium. Predominantly cytoplasmic but a small proportion colocalizes with SSX2 in the nucleus. Activation of protein kinase C results in redistribution to the periphery of lamellipodia. In the cytoskeleton, localizes to cortical actin.
Tissue Specificity
Expressed in brain, kidney, heart, pancreas and placenta. Not detected in skeletal muscle or liver.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Similarity
Pig (100%), Zebrafish (92%), Bovine (100%), Horse (92%), Rabbit (92%), Dog (92%), Chicken (92%), Xenopus (92%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9602219 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602219 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of RAB3IP using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis of RAB3IP using K562 whole cell lysates)
Related Product Information for anti-RAB3IP antibody
Function: Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.
Subunit Structure: Interacts with the N-terminal region of SSX2 (PubMed:12007189). Interacts with the GDP-bound forms of RAB3A, RAB3D, RAB8A and RAB8B (PubMed:12221131). Interacts with DCDC5 (PubMed:22159412).
Similarity: Belongs to the SEC2 family.
Subunit Structure: Interacts with the N-terminal region of SSX2 (PubMed:12007189). Interacts with the GDP-bound forms of RAB3A, RAB3D, RAB8A and RAB8B (PubMed:12221131). Interacts with DCDC5 (PubMed:22159412).
Similarity: Belongs to the SEC2 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 53 kDa
Predicted: 54 kDa
Predicted: 54 kDa
NCBI Official Full Name
rab-3A-interacting protein isoform A
NCBI Official Synonym Full Names
RAB3A interacting protein
NCBI Official Symbol
RAB3IP
NCBI Official Synonym Symbols
RABIN3; RABIN8
NCBI Protein Information
rab-3A-interacting protein
UniProt Protein Name
Rab-3A-interacting protein
Protein Family
UniProt Gene Name
RAB3IP
UniProt Synonym Gene Names
Rab3A-interacting protein
Uniprot Description
Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.
Research Articles on RAB3IP
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Product Notes
The RAB3IP rab3ip (Catalog #AAA9602219) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB3IP Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB3IP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000 IHC: 1:200. Researchers should empirically determine the suitability of the RAB3IP rab3ip for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB3IP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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