Rabbit anti-Human, Rat RAB35 Polyclonal Antibody | anti-RAB35 antibody

RAB35, CT (Ras-related Protein Rab-35, GTP-binding Protein RAY, Ras-related Protein Rab-1C, RAB1C, RAY)

Cat. Num. AAA647382

• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RAB35; Polyclonal Antibody; CT (Ras-related Protein Rab-35; GTP-binding Protein RAY; Ras-related Protein Rab-1C; RAB1C; RAY); Anti -RAB35; anti-RAB35 antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human RAB35. Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.

• Applicable Applications for anti-RAB35 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1ug/ml; Immunohistochemistry: 1:500
• Immunogen: Synthetic linear peptide corresponding to C-terminal of human RAB35 (KLH).
• Positive Control: Human brain tissue lysate
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-RAB35 antibody

RAB35 is a member of the Rab GTPases identified in the human genome. It is ubiquitously expressed and regulates a fast endocytotic recycling events associated with the terminal steps of cytokinesis, the formation of immunological synapse, and peptide loading onto major histocompatibility complex III molecules. RAB35 also plays a significant role in the formation of the immunological synapse (IS) between peptide-loaded antigen presenting cells (APCs) and T cells.

Product Categories/Family for anti-RAB35 antibody

Antibodies; Abs to GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 49456327
• UniProt Accession #: Q15286; B2R6E0; B4E390
• Molecular Weight: 23,025 Da
• NCBI Official Full Name: RAB35
• UniProt Protein Name: Ras-related protein Rab-35
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB35
• UniProt Synonym Gene Names: RAB1C; RAY
• UniProt Entry Name: RAB35_HUMAN

Uniprot Description

Function: In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge. Ref.9

Subunit structure: Interacts with DENND1A and DENND1B in a nucleotide-dependent manner. The low binding to DENND1C is nucleotide-independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein. Ref.11 Ref.13 Ref.14

Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side

Potential. Membrane › clathrin-coated pit. Cytoplasmic vesicle › clathrin-coated vesicle. Endosome. Melanosome. Note: Present on sorting endosomes and endosomal tubules. Tends to be enriched in PIP2-positive cell membrane domains. During mitosis, associated with the plasma membrane and present at the ingressing furrow during early cytokinesis as well as at the intercellular bridge later during cytokinesis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Ref.9 Ref.10

Post-translational modification: AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB35 constitutively active.Phosphocholinated by L.pneumophila AnkX. Both GDP-bound and GTP-bound forms can be phosphocholinated. Phosphocholination inhibits the GEF activity of DENND1A.

Sequence similarities: Belongs to the small GTPase superfamily. Rab family.