Immunohistochemistry (IHC)
(Human Colon MALT and Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit RAB31 Polyclonal Antibody | anti-RAB31 antibody
Anti-RAB31 Antibody (Internal)
Predicted: Mouse, Bovine, Rat, Pig
Predicted: Mouse, Bovine, Rat, Pig
IHC-P: 1:100
WB: 1:500-1:1000
Aliquot to avoid freeze/thaw cycles.
Immunohistochemistry (IHC)
(Human Colon MALT and Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Colon MALT and Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescent analysis of RAB31 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of RAB31 staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Western Blot (WB)
(Western blot analysis of RAB31 expression in MCF7 (A); Raw264.7 (B); rat brain (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of RAB31 expression in MCF7 (A); Raw264.7 (B); rat brain (C) whole cell lysates.)
NCBI and Uniprot Product Information
NCBI Description
Small GTP-binding proteins of the RAB family, such as RAB31, play essential roles in vesicle and granule targeting (Bao et al., 2002 [PubMed 11784320]).[supplied by OMIM, Jul 2009]
Uniprot Description
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Required for the integrity and for normal function of the Golgi apparatus and the trans-Golgi network. Plays a role in insulin-stimulated translocation of GLUT4 to the cell membrane. Plays a role in M6PR transport from the trans-Golgi network to endosomes. Plays a role in the internalization of EGFR from the cell membrane into endosomes. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis.
Research Articles on RAB31
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Product Notes
The RAB31 rab31 (Catalog #AAA2401691) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAB31 Antibody (Internal) reacts with Human Predicted: Mouse, Bovine, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's RAB31 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). ICC: 1:100-1:500 IHC-P: 1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the RAB31 rab31 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB31, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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