Mouse Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA Kit | P-ERK1/2 elisa kit

Mouse Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA Kit

Cat. Num. AAA728243

• Reactivity: Mouse
• Synonyms: Phosphorylation Extracellular signal Regulated Kinase 1/2; Mouse Phosphorylation Extracellular signal Regulated Kinase 1/2 ELISA Kit; P-ERK1/2 elisa kit

• Reactivity: Mouse

• Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma
• Assay Type: Sandwich
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for P-ERK1/2 elisa kit

For Samples: Cell culture fluid, body fluid, tissue homogenate, serum and blood plasma

Intended Uses: This P-ERK1/2 ELISA kit is intended for Laboratory research use only.The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of P-ERK1/2 in the sample, this P-ERK1/2 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus P-ERK1/2 concentration. The concentration of P-ERK1/2 in the samples is then determined by comparing the O.D. of the samples to the standard Curve.

Principle of the Assay: This P-ERK1/2 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a multiclonal antibody specific for P-ERK1/2. Standards or samples are then added to the microtiter plate wells and P-ERK1/2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of P-ERK1/2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for P-ERK1/2 are added to each well to "sandwich" the P-ERK1/2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain P-ERK1/2 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for P-ERK1/2 elisa kit

Signal Transduction