Rabbit anti-Human RAB25 Polyclonal Antibody | anti-RAB25 antibody

RAB25 (Ras-related Protein Rab-25, CATX-8, CATX8) (AP)

Cat. Num. AAA6270067

• Gene Names: RAB25; CATX-8; RAB11C
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: RAB25; Polyclonal Antibody; RAB25 (Ras-related Protein Rab-25; CATX-8; CATX8) (AP); anti-RAB25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RAB25. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-RAB25 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:600; WB: 0.05ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic linear peptide corresponding to human RAB25 (KLH). Species sequence homology: bovine and equine (100%).
• Conjugate: AP
• Positive Control: MCF7 cell lysate
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Categories/Family for anti-RAB25 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

1. Casanova, J.E., et al. (1999). Mol Biol Cell. 10:47-61. 2. Calhoun, B.C. and Goldenring, J.R. (1997). Biochem J. 325:559-564. 3. Goldenring, J. R., et al. (1996). Am J Physiol 270(33):G515-G525.

NCBI and Uniprot Product Information

• NCBI GI #: 116812567
• NCBI GeneID: 57111
• NCBI Accession #: NP_065120
• NCBI GenBank Nucleotide #: NM_020387
• UniProt Accession #: P57735
• Molecular Weight: 23kDa
• NCBI Official Full Name: ras-related protein Rab-25
• NCBI Official Synonym Full Names: RAB25, member RAS oncogene family
• NCBI Official Symbol: RAB25
• NCBI Official Synonym Symbols: CATX-8; RAB11C
• NCBI Protein Information: ras-related protein Rab-25
• UniProt Protein Name: Ras-related protein Rab-25
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB25
• UniProt Synonym Gene Names: CATX8
• UniProt Entry Name: RAB25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the RAS superfamily of small GTPases. The encoded protein is involved in membrane trafficking and cell survival. This gene has been found to be a tumor suppressor and an oncogene, depending on the context. Two variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

Function: Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Increases the rates of ovarian and breast cancers progression and aggressiveness by modulating cellular processes such as proliferation, survival and migration of epithelial tumor cells. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis

By similarity. Ref.7 Ref.8

Subunit structure: Interacts with RAB11FIP1, RAB11FIP2, RAB11FIP3 and RAB11FIP4. Interacts (via the hypervariable C-terminal region) with ITGB1 (via the cytoplasmic region); the interaction is GTP-dependent. Interacts with ITGAV. Associates with the integrin alpha-V/beta-1 heterodimer. Ref.6 Ref.8

Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side

Potential. Cell projection › pseudopodium membrane. Cytoplasmic vesicle. Note: Colocalizes with integrin alpha-V/beta-1 in vesicles at the pseudopodial tips.

Tissue specificity: Expressed in ovarian epithelium (NOE) and breast tissue. Expressed in ovarian cancer; expression is increased relative to NOE cells. Expression in ovarian cancer is stage dependent, with stage III and stage IV showing higher levels than early stage cancers. Expressed in breast cancer; expression is increased relative to normal breast tissue. Ref.7

Sequence similarities: Belongs to the small GTPase superfamily. Rab family.

Sequence caution: The sequence AAF98238.1 differs from that shown. Reason: Frameshift at position 196. The sequence AAM69362.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.