Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Human RAB1A Polyclonal Antibody | anti-RAB1A antibody
Polyclonal Antibody to RAB1A, Member RAS Oncogene Family (RAB1A)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. [provided by RefSeq, Oct 2008]
Uniprot Description
RAB1A: Probably required for transit of protein from the ER through Golgi compartment. Binds GTP and GDP and possesses intrinsic GTPase activity. Belongs to the small GTPase superfamily. Rab family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein; G protein, monomeric; G protein, monomeric, Rab
Chromosomal Location of Human Ortholog: 2p14
Cellular Component: cytosol; early endosome; endoplasmic reticulum membrane; extracellular exosome; Golgi apparatus; Golgi membrane; melanosome
Molecular Function: cadherin binding; GTP binding; GTPase activity; protein binding
Biological Process: autophagosome assembly; autophagy; cargo loading into COPII-coated vesicle; cell migration; COPII vesicle coating; defense response to bacterium; endocytosis; ER to Golgi vesicle-mediated transport; establishment of endothelial intestinal barrier; Golgi organization and biogenesis; growth hormone secretion; interleukin-8 secretion; melanosome transport; positive regulation of ubiquitin protein ligase activity; post-translational protein modification; retrograde vesicle-mediated transport, Golgi to ER; vesicle transport along microtubule; vesicle-mediated transport; virus assembly
Research Articles on RAB1A
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Product Notes
The RAB1A rab1a (Catalog #AAA2033992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to RAB1A, Member RAS Oncogene Family (RAB1A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the RAB1A rab1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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