Goat RAB1A Polyclonal Antibody | anti-RAB1A antibody

Anti-RAB1A Antibody (C-Terminus) IHC-plus

Cat. Num. AAA249833

• Gene Names: RAB1A; RAB1; YPT1
• Reactivity: Mouse, Rat, Human, Monkey; Non-Reactive Species: Canine
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Immunoaffinity purified
• Synonyms: RAB1A; Polyclonal Antibody; Anti-RAB1A Antibody (C-Terminus) IHC-plus; Goat Polyclonal (IgG) to Mouse RAB1A; Mouse RAB1A; GTP binding protein Rab1a; YPT1-related protein; Rab GTPase YPT1 homolog; RAB1; Ras-related protein Rab-1A; YPT1; anti-RAB1A antibody

• Host: Goat
• Reactivity: Mouse, Rat, Human, Monkey; Non-Reactive Species: Canine
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects total Rab1 protein by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab1a and GFP-Rab1b cds.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 20% glycerol, 0.05% sodium azide.
• Concentration: 3 mg/ml (varies by lot)
• Sequence Length: 205

• Applicable Applications for anti-RAB1A antibody: Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF (1:100 - 1:250); IHC-P (5 ug/ml); WB (1:250 - 1:2000)
• Immunogen: RAB1A antibody was raised against purified recombinant peptides derived from within residues 110 aa to the C-terminus of mouse Rab1a and Rab1b produced in E Coli.
• Immunogen Description: Purified recombinant peptides derived from within residues 110 aa to the C-terminus of mouse Rab1a and Rab1b produced in E Coli.
• Immunogen Type: Recombinant protein
• Antigen Modification: C-Terminus
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-RAB1A / RAB1 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-RAB1A / RAB1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence - anti-Rab1 antibody in COS-7 cells at 1:50 dilution. Cells were fixed with 4% of PFA.)

Western Blot (WB)

(Western blot. Anti-Rab1 antibody at 1:1000 dilution. 293HEK transfected lysate at 50 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.)

Related Product Information for anti-RAB1A antibody

RAB1A Antibody, GTP binding protein Rab1a Antibody, YPT1-related protein Antibody, Rab GTPase YPT1 homolog Antibody, RAB1 Antibody, Ras-related protein Rab-1A Antibody, YPT1 Antibody Description: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion.

NCBI and Uniprot Product Information

• NCBI GI #: 4758988
• NCBI GeneID: 5861
• NCBI Accession #: NP_004152.1
• NCBI GenBank Nucleotide #: NM_004161.4
• UniProt Accession #: P62820; P11476; Q6FIE7; Q96N61; Q9Y3T2
• Molecular Weight: 13,903 Da
• NCBI Official Full Name: ras-related protein Rab-1A isoform 1
• NCBI Official Synonym Full Names: RAB1A, member RAS oncogene family
• NCBI Official Symbol: RAB1A
• NCBI Official Synonym Symbols: RAB1; YPT1
• NCBI Protein Information: ras-related protein Rab-1A
• UniProt Protein Name: Ras-related protein Rab-1A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB1A
• UniProt Synonym Gene Names: RAB1
• UniProt Entry Name: RAB1A_HUMAN

NCBI Description

This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

RAB1A: Probably required for transit of protein from the ER through Golgi compartment. Binds GTP and GDP and possesses intrinsic GTPase activity. Belongs to the small GTPase superfamily. Rab family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 2p14

Cellular Component: cytosol; early endosome; endoplasmic reticulum membrane; Golgi apparatus; Golgi membrane; melanosome

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: autophagic vacuole formation; autophagy; cell migration; cellular protein metabolic process; cilium biogenesis; COPII coating of Golgi vesicle; defense response to bacterium; endocytosis; ER to Golgi vesicle-mediated transport; Golgi organization and biogenesis; growth hormone secretion; melanosome transport; mitotic cell cycle; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; small GTPase mediated signal transduction; vesicle transport along microtubule; vesicle-mediated transport; virus assembly