Western Blot (WB)
(Western blotAll lanes :RAB12 antibody at 2 ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 27 kDaObserved band size: 27,42 kDa)
Rabbit anti-Human RAB12 Polyclonal Antibody | anti-RAB12 antibody
RAB12 Antibody
Reactivity
Human
Applications
ELISA, Western Blot, Immunofluorescence
Purity
>95%, Protein G purified
Synonyms
RAB12; Polyclonal Antibody; RAB12 Antibody; Ras-related protein Rab-12; anti-RAB12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%, Protein G purified
Form/Format
Liquid
Sequence Length
244
Applicable Applications for anti-RAB12 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Species
Human
Immunogen
Recombinant human Ras-related protein Rab-12 protein
Conjugate
Non-conjugated
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-85109 / sc-85110
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western blotAll lanes :RAB12 antibody at 2 ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 27 kDaObserved band size: 27,42 kDa)
Western Blot (WB)
(Western blotAll lanes :RAB12 antibody at 2 ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 27 kDaObserved band size: 27,42 kDa)
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells using MBS7045059 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of Hela cells using MBS7045059 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-RAB12 antibody
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,248 Da
NCBI Official Full Name
ras-related protein Rab-12
NCBI Official Synonym Full Names
RAB12, member RAS oncogene family
NCBI Official Symbol
RAB12
NCBI Protein Information
ras-related protein Rab-12
UniProt Protein Name
Ras-related protein Rab-12
Protein Family
UniProt Gene Name
RAB12
UniProt Entry Name
RAB12_HUMAN
Uniprot Description
RAB12: Belongs to the small GTPase superfamily. Rab family.
Protein type: G protein, monomeric, Rab; G protein, monomeric; G protein
Chromosomal Location of Human Ortholog: 18p11.22
Cellular Component: lysosome; recycling endosome membrane
Molecular Function: GDP binding
Biological Process: cellular protein catabolic process; endosome to lysosome transport
Research Articles on RAB12
Similar Products
Product Notes
The RAB12 rab12 (Catalog #AAA7045059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB12 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB12 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). Researchers should empirically determine the suitability of the RAB12 rab12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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