Rabbit anti-Human RAB11FIP2 Polyclonal Antibody | anti-RAB11FIP2 antibody

RAB11FIP2, ID (RAB11FIP2, KIAA0941, Rab11 family-interacting protein 2, NRip11) (Azide free) (HRP)

Cat. Num. AAA6339529

• Gene Names: RAB11FIP2; nRip11; Rab11-FIP2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RAB11FIP2; Polyclonal Antibody; ID (RAB11FIP2; KIAA0941; Rab11 family-interacting protein 2; NRip11) (Azide free) (HRP); NRip11; anti-RAB11FIP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RAB11FIP2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: RAB11FIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-382 amino acids from the Central region of human RAB11FIP2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Wwestern blot analysis using MBS6002737 in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the RAB11FIP2 antibody detected the RAB11FIP2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using MBS6002737 in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MDA-MB231 cells (right histogram) using MBS6002737 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-RAB11FIP2 antibody

RAB11FIP2 is an adapter protein that plays a role in the secretory pathway. It is thought to be important for endosome recycling and receptor-mediated endocytosis. In endosome recycling, RAB11-FIP2 regulates vesicle transport from the endosomal recycling compartment (ERC) to the plasma membrane.

Product Categories/Family for anti-RAB11FIP2 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Wang, Z., et al. Cell 135(3):535-548(2008). Utley, T.J., et al. Proc. Natl. Acad. Sci. U.S.A. 105(29):10209-10214(2008). Ducharme, N.A., et al. Am. J. Physiol., Cell Physiol. 293 (3), C1059-C1072 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 7662394
• NCBI GeneID: 22841
• NCBI Accession #: NP_055719.1
• NCBI GenBank Nucleotide #: NM_014904.2
• UniProt Accession #: Q7L804; Q9Y2F0; A6NEI4
• Molecular Weight: 58,279 Da
• NCBI Official Full Name: rab11 family-interacting protein 2
• NCBI Official Synonym Full Names: RAB11 family interacting protein 2 (class I)
• NCBI Official Symbol: RAB11FIP2
• NCBI Official Synonym Symbols: nRip11; Rab11-FIP2
• NCBI Protein Information: rab11 family-interacting protein 2; RAB11-FIP2 long isoform
• UniProt Protein Name: Rab11 family-interacting protein 2
• Protein Family: Rab11 family-interacting protein
• UniProt Gene Name: RAB11FIP2
• UniProt Synonym Gene Names: KIAA0941; Rab11-FIP2
• UniProt Entry Name: RFIP2_HUMAN

Uniprot Description

RAB11FIP2: a Rab11 effector protein acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Binds preferentially to phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA). Translocates with RAB11A from the vesicles of the endocytic recycling compartment (ERC) to the plasma membrane. Phosphorylation by MARK2 may affect the establishment of epithelial polarity.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: nucleoplasm; cytoplasmic vesicle membrane; intracellular membrane-bound organelle; recycling endosome membrane; plasma membrane; endosome

Molecular Function: protein binding; protein homodimerization activity; protein kinase binding; Rab GTPase binding

Biological Process: regulated secretory pathway; establishment of cell polarity; water transport; renal water homeostasis; transmembrane transport