Testing Data
anti-Human, Mouse IL-10Ralpha Antibody | anti-IL-10Ralpha antibody
IL-10Ralpha (Ab-496) Antibody
Gene Names
IL10RA; CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
IL-10Ralpha; IL-10Ralpha (Ab-496) Antibody; I10R; I10R1; IL-10R alpha; IL-10R1; IL10R; IL10RA; Interleukin-10 receptor alpha chain precursor; anti-IL-10Ralpha antibody
Reactivity
Human, Mouse
Specificity
IL-10Ralpha (Ab-496) antibody detects endogenous levels of total IL-10Ralpha protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
578
Applicable Applications for anti-IL-10Ralpha antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:10000
IF: 1:100~1:500
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human IL-10Ralpha around the phosphorylation site of tyrosine 496 (K-G-YP-L-K).
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
Testing Data
Western Blot (WB)
(Peptide-+ Western blot analysis of extracts from HepG2 cells, treated with Na2VO3 (0.3nM, 40mins), using IL-10Ralpha (Ab-496) antibody. Immunofluorescence analysis of HepG2 cells, using IL-10Ralpha (Ab-496) antibody.)
Western Blot (WB)
(Peptide-+ Western blot analysis of extracts from HepG2 cells, treated with Na2VO3 (0.3nM, 40mins), using IL-10Ralpha (Ab-496) antibody. Immunofluorescence analysis of HepG2 cells, using IL-10Ralpha (Ab-496) antibody.)
References
Liu Y., J. Immunol. 152:1821-1829(1994). Zhang Z., Protein Sci. 13:2819-2824(2004). Josephson K., Immunity 15:35-46(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,003 Da
NCBI Official Full Name
interleukin-10 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 10 receptor subunit alpha
NCBI Official Symbol
IL10RA
NCBI Official Synonym Symbols
CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1
NCBI Protein Information
interleukin-10 receptor subunit alpha
UniProt Protein Name
Interleukin-10 receptor subunit alpha
UniProt Gene Name
IL10RA
UniProt Synonym Gene Names
IL10R; IL-10 receptor subunit alpha; IL-10R subunit alpha; IL-10RA; IL-10R subunit 1; IL-10R1
NCBI Description
The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]
Uniprot Description
Receptor for IL10; binds IL10 with a high affinity.
Research Articles on IL-10Ralpha
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Product Notes
The IL-10Ralpha il10ra (Catalog #AAA8509741) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The IL-10Ralpha (Ab-496) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-10Ralpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the IL-10Ralpha il10ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-10Ralpha, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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