Rabbit Rab9 Monoclonal Antibody | anti-Rab9 antibody

Rab9 Rabbit Monoclonal Antibody

Cat. Num. AAA3014079

• Gene Names: RAB9A; RAB9
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein A affinity purified
• Synonyms: Rab9; Monoclonal Antibody; Rab9 Rabbit Monoclonal Antibody; Ras-related protein Rab-9A; RAB9A; RAB9; anti-Rab9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of Rab9 and does not cross-react with related proteins.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 201

• Applicable Applications for anti-Rab9 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000-1:2000; ICC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Rab9 Rabbit mAb at 1:1000 dilutionLane1:HepG2 whole cell lysateLane2:K562 whole cell lysate)

Immunocytochemistry (ICC)

(ICC staining Rab9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Rab9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS)

Related Product Information for anti-Rab9 antibody

The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the SEC4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. At least eight members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 1710003
• NCBI GeneID: 9367
• UniProt Accession #: P51151
• Molecular Weight: ~ 23kDa
• NCBI Official Full Name: Ras-related protein Rab-9A
• NCBI Official Synonym Full Names: RAB9A, member RAS oncogene family
• NCBI Official Symbol: RAB9A
• NCBI Official Synonym Symbols: RAB9
• NCBI Protein Information: ras-related protein Rab-9A
• UniProt Protein Name: Ras-related protein Rab-9A
• Protein Family: Rab9 effector protein with kelch motifs
• UniProt Gene Name: RAB9A
• UniProt Synonym Gene Names: RAB9
• UniProt Entry Name: RAB9A_HUMAN

Uniprot Description

RAB9A: Involved in the transport of proteins between the endosomes and the trans Golgi network. Interacts (preferentially in its GTP-bound form) with GCC2 (via its GRIP domain). Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; G protein, monomeric, Rab; G protein, monomeric

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: Golgi membrane; phagocytic vesicle membrane; endoplasmic reticulum membrane; lysosome; late endosome; vacuolar membrane; plasma membrane; phagocytic vesicle

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: intracellular protein transport; metabolic process; Rab protein signal transduction; retrograde transport, endosome to Golgi; positive regulation of exocytosis