Rabbit anti-Human, Mouse Pyruvate Kinase, Isozymes M1/M2 Polyclonal Antibody | anti-PKM2 antibody

Pyruvate Kinase, Isozymes M1/M2, CT (L398) (PKM2, Pyruvate Kinase Muscle Isozyme, Cytosolic Thyroid Hormone-binding Protein, CTHBP, Opa-interacting Protein 3, OIP-3, Pyruvate Kinase 2/3, Thyroid Hormone-binding Protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP

Cat. Num. AAA6496319

• Gene Names: PKM; PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Pyruvate Kinase; Isozymes M1/M2; Polyclonal Antibody; CT (L398) (PKM2; Pyruvate Kinase Muscle Isozyme; Cytosolic Thyroid Hormone-binding Protein; CTHBP; Opa-interacting Protein 3; OIP-3; Pyruvate Kinase 2/3; Thyroid Hormone-binding Protein 1; THBP1; Tumor M2-PK; p58; PKM; OIP; anti-PKM2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PKM1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 531

• Applicable Applications for anti-PKM2 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human PKM1 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PKM1 antibody (C-term L398) in Hela cell line lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of PKM1 antibody (C-term L398) in mouse testis tissue lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PKM1 Antibody (C-term L398), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-PKM2 antibody

Antibodies; Protein Kinases

References

1. Dombrauckas J.D.Biochemistry 44:9417-9429(2005). 2. Daub H., Olsen J.V.Mol. Cell 31:438-448(2008). 3. Ashizawa K., McPhie P.Biochemistry 30:7105-7111(1991).

NCBI and Uniprot Product Information

• NCBI GI #: 33286420
• NCBI GeneID: 5315
• NCBI Accession #: NP_872270
• NCBI GenBank Nucleotide #: NP_872270.1
• UniProt Accession #: P14618
• Molecular Weight: 57.937
• NCBI Official Full Name: pyruvate kinase PKM isoform b
• NCBI Official Synonym Full Names: pyruvate kinase M1/2
• NCBI Official Symbol: PKM
• NCBI Official Synonym Symbols: PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
• NCBI Protein Information: pyruvate kinase PKM
• UniProt Protein Name: Pyruvate kinase PKM
• UniProt Gene Name: PKM
• UniProt Synonym Gene Names: OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
• UniProt Entry Name: KPYM_HUMAN

NCBI Description

This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]