Western Blot (WB)
(Western blot analysis of PKM1 antibody (C-term L398) in Hela cell line lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.)
Rabbit anti-Human, Mouse Pyruvate Kinase, Isozymes M1/M2 Polyclonal Antibody | anti-PKM2 antibody
Pyruvate Kinase, Isozymes M1/M2, CT (L398) (PKM2, Pyruvate Kinase Muscle Isozyme, Cytosolic Thyroid Hormone-binding Protein, CTHBP, Opa-interacting Protein 3, OIP-3, Pyruvate Kinase 2/3, Thyroid Hormone-binding Protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP
Gene Names
PKM; PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Reactivity
Human, Mouse
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
Pyruvate Kinase; Isozymes M1/M2; Polyclonal Antibody; CT (L398) (PKM2; Pyruvate Kinase Muscle Isozyme; Cytosolic Thyroid Hormone-binding Protein; CTHBP; Opa-interacting Protein 3; OIP-3; Pyruvate Kinase 2/3; Thyroid Hormone-binding Protein 1; THBP1; Tumor M2-PK; p58; PKM; OIP; anti-PKM2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PKM1. Species Crossreactivity: mouse.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Sequence Length
531
Applicable Applications for anti-PKM2 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human PKM1 (KLH).
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of PKM1 antibody (C-term L398) in Hela cell line lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PKM1 antibody (C-term L398) in Hela cell line lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PKM1 antibody (C-term L398) in mouse testis tissue lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of PKM1 antibody (C-term L398) in mouse testis tissue lysates (35ug/lane). PKM1 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PKM1 Antibody (C-term L398), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with PKM1 Antibody (C-term L398), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Product Categories/Family for anti-PKM2 antibody
References
1. Dombrauckas J.D.Biochemistry 44:9417-9429(2005). 2. Daub H., Olsen J.V.Mol. Cell 31:438-448(2008). 3. Ashizawa K., McPhie P.Biochemistry 30:7105-7111(1991).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57.937
NCBI Official Full Name
pyruvate kinase PKM isoform b
NCBI Official Synonym Full Names
pyruvate kinase M1/2
NCBI Official Symbol
PKM
NCBI Official Synonym Symbols
PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
NCBI Protein Information
pyruvate kinase PKM
UniProt Protein Name
Pyruvate kinase PKM
UniProt Gene Name
PKM
UniProt Synonym Gene Names
OIP3; PK2; PK3; PKM2; CTHBP; OIP-3; THBP1
UniProt Entry Name
KPYM_HUMAN
NCBI Description
This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
Research Articles on PKM2
Similar Products
Product Notes
The PKM2 pkm (Catalog #AAA6496311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pyruvate Kinase, Isozymes M1/M2, CT (L398) (PKM2, Pyruvate Kinase Muscle Isozyme, Cytosolic Thyroid Hormone-binding Protein, CTHBP, Opa-interacting Protein 3, OIP-3, Pyruvate Kinase 2/3, Thyroid Hormone-binding Protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Pyruvate Kinase, Isozymes M1/M2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PKM2 pkm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyruvate Kinase, Isozymes M1/M2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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