Rabbit CAMK2A Polyclonal Antibody | anti-CAMK2A antibody

CAMK2A (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha, CaM-kinase II Subunit alpha, CaM Kinase II Subunit alpha, CAMKA, KIAA0968) (MaxLight 405)

Cat. Num. AAA6444594

• Gene Names: CAMK2A; CAMKA
• Reactivity: Rat, Bovine, Chicken, Guinea Pig, Hamster, Human, Mouse, Rabbit
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: CAMK2A; Polyclonal Antibody; CAMK2A (Calcium/Calmodulin-dependent Protein Kinase Type II Subunit alpha; CaM-kinase II Subunit alpha; CaM Kinase II Subunit alpha; CAMKA; KIAA0968) (MaxLight 405); anti-CAMK2A antibody

• Host: Rabbit
• Reactivity: Rat, Bovine, Chicken, Guinea Pig, Hamster, Human, Mouse, Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat CaMK II. Species Crossreactivity: human, mouse, bovine, hamster, guinea pig, chicken and rabbit.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-CAMK2A antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to the N-terminal of rat CaMK II.
• Conjugate: MaxLight405
• Control: HEK293 cell lysate
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot Analysis: HEK293 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-CaM Kinase II (1:500). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein CAMKII (~60kD).)

Western Blot (WB)

(Western Blot Analysis: Representative image from a previous lot. Jurkat lysate (Lane1), RAW 264 lysate (Lane2), HEK293 lysate (Lane 3), NIH/3T3 lysate (Lane 4), C2C12 lysate (Lane 5), Huvec lysate (Lane 6), A431 lysate (Lane 7), L6 lysate (Lane 8), HepG2 lysate (Lane 9), HeLa lysate (Lane 10) and PC3 lysate (Lane 11) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-CaM Kinase II (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein CaM Kinase II (pan-isoforms) (~50-72kD).)

Product Categories/Family for anti-CAMK2A antibody

Antibodies; Calmodulin

References

1. Downregulation of delta CaM kinase II in human tumor cells.Tombes, R M, et al. (1999). Biochim. Biophys. Acta, 1452: 1-11 (1999). 2. Regulation of DLG localization at synapses by CaMKII-dependent phosphorylation. Koh, Y H, et al. (1999). Cell, 98: 353-63 (1999). 3. Inhibition of calcium/calmodulin-dependent protein kinase II in rat hippocampus attenuates morphine tolerance and dependence. Fan, G H, et al. (1999). Mol. Pharmacol., 56: 39-45 (1999). 4. Identification and expression of delta-isoforms of the multifunctional Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human myocardium. Hoch, B, et al. (1999). Circ. Res., 84: 713-21 (1999). 5. Regional distribution of type II Ca2+/calmodulin-dependent protein kinase in rat brain. Erondu, N E and Kennedy, M B (1985) J. Neurosci., 5: 3270-7 (1985). 6. AlphaCaMKII binding to the C-terminal tail of NMDA receptor subunit NR2A and its modulation by autophosphorylation. Gardoni, F, et al. (1999). FEBS Lett., 456: 394-8 (1999). 7. CaMKII regulates the density of central glutamatergic synapses in vivo. Rongo, C and Kaplan, J M (1999). Nature, 402: 195-9 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 25952118
• NCBI GeneID: 815
• NCBI Accession #: NP_741960.1
• NCBI GenBank Nucleotide #: NM_171825.2
• UniProt Accession #: Q9UQM7; Q9UL21; Q9Y2H4; Q9Y352
• Molecular Weight: 54,088 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2
• NCBI Official Synonym Full Names: calcium/calmodulin-dependent protein kinase II alpha
• NCBI Official Symbol: CAMK2A
• NCBI Official Synonym Symbols: CAMKA
• NCBI Protein Information: calcium/calmodulin-dependent protein kinase type II subunit alpha; CaMKIINalpha; CaMK-II alpha subunit; caMK-II subunit alpha; CaM-kinase II alpha chain; CaM kinase II alpha subunit; caM kinase II subunit alpha; calcium/calmodulin-dependent protein kinase
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit alpha
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2A
• UniProt Synonym Gene Names: CAMKA; KIAA0968; CaM kinase II subunit alpha
• UniProt Entry Name: KCC2A_HUMAN

NCBI Description

The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

CAMK2A: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, CAMK; EC 2.7.11.17; Protein kinase, Ser/Thr (non-receptor); CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 5q32

Cellular Component: nucleoplasm; presynaptic membrane; mitochondrion; plasma membrane; cytosol; cell junction; nucleus

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; glutamate receptor binding; protein binding; protein homodimerization activity; calmodulin-dependent protein kinase activity; kinase activity; ATP binding

Biological Process: synaptic transmission; peptidyl-serine phosphorylation; protein amino acid autophosphorylation; calcium ion transport; cytokine and chemokine mediated signaling pathway; regulation of neurotransmitter secretion; positive regulation of calcium ion transport; protein amino acid phosphorylation; regulation of neuronal synaptic plasticity; G1/S transition of mitotic cell cycle; activation of NF-kappaB transcription factor