Rabbit anti-Human, Mouse PYGM Polyclonal Antibody | anti-PYGM antibody

PYGM Antibody

Cat. Num. AAA9405697

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification.
• Synonyms: PYGM; Polyclonal Antibody; PYGM Antibody; Myophosphorylase; phosphorylase; glycogen; muscle; anti-PYGM antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total PYGM protein.
• Purity/Purification: Antigen affinity purification.
• Form/Format: Rabbit IgG in pH7.3 PBS, 0.05% NaN3, 50% Glycerol.
• Concentration: 1.6 mg/ml (varies by lot)
• Sequence Length: 754

• Applicable Applications for anti-PYGM antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Western blotting: 1:500-1:2000; Immunohistochemistry: 1:30-1:150
• Immunogen Type: Recombinant Protein
• Immunogen Description: Fusion protein corresponding to residues near the C terminal of human phosphorylase, glycogen, muscle
• Target Name: PYGM
• Preparation and Storage: Store at -20 degree C

Testing Data

(Gel: 8%SDS-PAGE Lysates (from left to right): Mouse heart tissue Amount of lysate: 40ug per lane Primary antibody: 1/400 dilution Secondary antibody dilution: 1/8000 Exposure time: 40 seconds)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue using at dilution 1/30.)

Related Product Information for anti-PYGM antibody

This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-PYGM antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 257900462
• NCBI GeneID: 5837
• NCBI Accession #: NP_001158188.1
• NCBI GenBank Nucleotide #: NM_001164716.1
• UniProt Accession #: P11217; A0AVK1; A6NDY6
• Molecular Weight: 87,317 Da
• NCBI Official Full Name: glycogen phosphorylase, muscle form isoform 2
• NCBI Official Synonym Full Names: phosphorylase, glycogen, muscle
• NCBI Official Symbol: PYGM
• NCBI Protein Information: glycogen phosphorylase, muscle form
• UniProt Protein Name: Glycogen phosphorylase, muscle form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: PYGM
• UniProt Entry Name: PYGM_HUMAN

NCBI Description

This gene encodes a muscle enzyme involved in glycogenolysis. Highly similar enzymes encoded by different genes are found in liver and brain. Mutations in this gene are associated with McArdle disease (myophosphorylase deficiency), a glycogen storage disease of muscle. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Sep 2009]

Uniprot Description

PYGM: an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Dimers associate into a tetramer to form the enzymatically active phosphorylase A. Phosphorylation of Ser-14 converts phosphorylase B (unphosphorylated) to phosphorylase A.

Protein type: Carbohydrate Metabolism - starch and sucrose; EC 2.4.1.1; Transferase; Endoplasmic reticulum; Phosphorylase

Chromosomal Location of Human Ortholog: 11q12-q13.2

Cellular Component: cytoplasm; cytosol; sarcoplasmic reticulum

Molecular Function: AMP binding; carbohydrate binding; drug binding; glycogen phosphorylase activity; protein binding; pyridoxal phosphate binding

Biological Process: carbohydrate metabolic process; cellular calcium ion homeostasis; glucose metabolic process; glycogen catabolic process; glycogen metabolic process; response to cAMP; response to hypoxia

Disease: Glycogen Storage Disease V