Immunohistochemistry (IHC)
(MBS9603279 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit PYCR1 Polyclonal Antibody | anti-PYCR1 antibody
PYCR1 Antibody
Gene Names
PYCR1; P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PYCR1; Polyclonal Antibody; PYCR1 Antibody; P5C; P5C reductase 1; P5C reductase; P5CR 1; P5CR; PIG45; PP222; Proliferation inducing protein 45; PYCR 1; PYCR; Pyrroline 5 carboxylate reductase 1; Pyrroline 5 carboxylate reductase 1 mitochondrial; anti-PYCR1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Zebrafish
Clonality
Polyclonal
Isotype
IgG
Specificity
PYCR1 antibody detects endogenous levels of PYCR1.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
319
Applicable Applications for anti-PYCR1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human PYCR1.
Subcellular Location
Mitochondrial
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603279 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603279 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts of HT-1080 cells, using PYCR1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of HT-1080 cells, using PYCR1 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse Myeloma cell, using PYCR1 antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse Myeloma cell, using PYCR1 antibody. Lane 1 was treated with the blocking peptide.)
Related Product Information for anti-PYCR1 antibody
Function: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.
Subunit Structure: Homodecamer; composed of 5 homodimers.
Similarity: Belongs to the pyrroline-5-carboxylate reductase family.
Subunit Structure: Homodecamer; composed of 5 homodimers.
Similarity: Belongs to the pyrroline-5-carboxylate reductase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 33 kDa; 35 kDa
Predicted: 34 kDa
Predicted: 34 kDa
NCBI Official Full Name
pyrroline-5-carboxylate reductase 1, mitochondrial isoform 1
NCBI Official Synonym Full Names
pyrroline-5-carboxylate reductase 1
NCBI Official Symbol
PYCR1
NCBI Official Synonym Symbols
P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
NCBI Protein Information
pyrroline-5-carboxylate reductase 1, mitochondrial
UniProt Protein Name
Pyrroline-5-carboxylate reductase 1, mitochondrial
Protein Family
UniProt Gene Name
PYCR1
UniProt Synonym Gene Names
P5C reductase 1; P5CR 1
NCBI Description
This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Uniprot Description
Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.
Research Articles on PYCR1
Similar Products
Product Notes
The PYCR1 pycr1 (Catalog #AAA9603279) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PYCR1 Antibody reacts with Human, Mouse, Rat, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's PYCR1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PYCR1 pycr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PYCR1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
