Rat Fibronectin ELISA Kit | FN1 elisa kit

Rat Fibronectin PicoKine ELISA Kit

Cat. Num. AAA175957

• Gene Names: Fn1; fn-1; FIBNEC
• Reactivity: Rat
• Synonyms: Fibronectin; Rat Fibronectin PicoKine ELISA Kit; fibronectin 1; CIG; Cold insoluble globulin; Fibronectin 1; FINC; FN; FN1; FNZ; Migration stimulating factor; MSF; FN1 elisa kit

• Reactivity: Rat
• Specificity: Natural rat Fibronectin
• Sequence Length: 2477

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: < 15pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Rat Fibronectin PicoKine ELISA Kit standard curve )

Related Product Information for FN1 elisa kit

Principle of the assay: rat Fibronectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Fibronectin has been precoated onto 96-well plates. Standards(from plasma) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Fibronectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Fibronectin amount of sample captured in plate.
Background: Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides.1 These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs andvarious compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris.2 Fibronectins are involved in celladhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.3 The standard used in this kit is isolated fromrat plasma with the molecular mass of 200-250KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 120178
• NCBI GeneID: 25661
• UniProt Accession #: P04937; Q6LDX9
• Molecular Weight: 259,350 Da
• NCBI Official Full Name: Fibronectin
• NCBI Official Synonym Full Names: fibronectin 1
• NCBI Official Symbol: Fn1
• NCBI Official Synonym Symbols: fn-1; FIBNEC
• NCBI Protein Information: fibronectin; FN
• UniProt Protein Name: Fibronectin
• Protein Family: Fibronectin
• UniProt Gene Name: Fn1
• UniProt Synonym Gene Names: FN
• UniProt Entry Name: FINC_RAT

NCBI Description

extracellular matrix component; may play a role in fibrosis and tumor metastasis [RGD, Feb 2006]

Uniprot Description

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Motility/polarity/chemotaxis; Cell adhesion; Secreted, signal peptide

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; fibrinogen complex; apical plasma membrane; extracellular region; ER-Golgi intermediate compartment; basement membrane; basal lamina

Molecular Function: heparin binding; integrin binding; protein binding; protease activator activity; protease binding; extracellular matrix structural constituent; mercury ion binding

Biological Process: integrin activation; extracellular matrix organization and biogenesis; ossification; wound healing; positive regulation of chemotaxis; positive regulation of axon extension; cell-matrix adhesion; response to glucocorticoid stimulus; glial cell migration; regulation of cell shape; cell activation; response to ozone; acute-phase response; response to wounding; calcium-independent cell-matrix adhesion; cell-substrate junction assembly; angiogenesis; peptide cross-linking; cell adhesion; positive regulation of cell migration; negative regulation of apoptosis