Rabbit anti-Human, Mouse PYCR1 Polyclonal Antibody | anti-PYCR1 antibody

PYCR1 Rabbit Polyclonal

Cat. Num. AAA7603202

• Gene Names: PYCR1; P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: PYCR1; Polyclonal Antibody; PYCR1 Rabbit Polyclonal; anti-PYCR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 346

• Applicable Applications for anti-PYCR1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Pyrroline-5-carboxylate reductase 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using MBS7603202 (PYCR1 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9) .)

Western Blot (WB)

(HT-1080 cells were subjected to SDS PAGE followed by western blot with MBS7603202 (PYCR1 Antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 533112491
• NCBI GeneID: 5831
• NCBI Accession #: NP_001269210.1
• NCBI GenBank Nucleotide #: NM_001282281.1
• Molecular Weight: 35,981 Da
• NCBI Official Full Name: pyrroline-5-carboxylate reductase 1, mitochondrial isoform 5
• NCBI Official Synonym Full Names: pyrroline-5-carboxylate reductase 1
• NCBI Official Symbol: PYCR1
• NCBI Official Synonym Symbols: P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
• NCBI Protein Information: pyrroline-5-carboxylate reductase 1, mitochondrial
• UniProt Protein Name: Pyrroline-5-carboxylate reductase 1, mitochondrial
• Protein Family: Pyrroline-5-carboxylate reductase
• UniProt Gene Name: PYCR1
• UniProt Synonym Gene Names: P5C reductase 1; P5CR 1
• UniProt Entry Name: P5CR1_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

PYCR1: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). A multisystem disorder characterized by the appearance of premature aging, wrinkled and lax skin with reduced elasticity, joint laxity, craniofacial dysmorphic features, intrauterine growth retardation with some degree of postnatal growth deficiency, and developmental delay. Defects in PYCR1 are the cause of cutis laxa, autosomal recessive, type 3B (ARCL3B). ARCL3B is a disorder characterized by an aged appearance with distinctive facial features, sparse hair, ophthalmologic abnormalities, intrauterine growth retardation, and cutis laxa. Belongs to the pyrroline-5-carboxylate reductase family.

Protein type: EC 1.5.1.2; Mitochondrial; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: identical protein binding; protein binding; pyrroline-5-carboxylate reductase activity

Biological Process: amino acid biosynthetic process; proline biosynthetic process; regulation of mitochondrial membrane potential

Disease: Cutis Laxa, Autosomal Recessive, Type Iib; Cutis Laxa, Autosomal Recessive, Type Iiib