Rabbit anti-Human PUS10 Polyclonal Antibody | anti-PUS10 antibody

PUS10, NT (PUS10, CCDC139, DOBI, Putative tRNA pseudouridine synthase Pus10, Coiled-coil domain-containing protein 139, tRNA pseudouridine 55 synthase, tRNA pseudouridylate synthase, tRNA-uridine isomerase) (MaxLight 405)

Cat. Num. AAA6339200

• Gene Names: PUS10; DOBI; CCDC139
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PUS10; Polyclonal Antibody; NT (PUS10; CCDC139; DOBI; Putative tRNA pseudouridine synthase Pus10; Coiled-coil domain-containing protein 139; tRNA pseudouridine 55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase) (MaxLight 405); tRNA-uridine isomerase; anti-PUS10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-PUS10 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PUS10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-78 amino acids from the N-terminal region of human PUS10.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Related Product Information for anti-PUS10 antibody

Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 [PubMed 17900615]).[supplied by OMIM].

Product Categories/Family for anti-PUS10 antibody

Antibodies; Enzymes; Isomerase

References

Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010): McGovern, D.P., et al. Nat. Genet. 42(4):332-337(2010). Dubois, P.C., et al. Nat. Genet. 42(4):295-302(2010). Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). Park, S.Y., et al. BMB Rep 42(8):511-515(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 207113130
• NCBI GeneID: 150962
• NCBI Accession #: NP_653310.2
• NCBI GenBank Nucleotide #: NM_144709.2
• UniProt Accession #: Q3MIT2; Q5JPJ5; Q96MI8
• Molecular Weight: 60,244 Da
• NCBI Official Full Name: putative tRNA pseudouridine synthase Pus10
• NCBI Official Synonym Full Names: pseudouridylate synthase 10
• NCBI Official Symbol: PUS10
• NCBI Official Synonym Symbols: DOBI; CCDC139
• NCBI Protein Information: putative tRNA pseudouridine synthase Pus10; psi55 synthase; tRNA-uridine isomerase; pseudouridine synthase 10; tRNA pseudouridylate synthase; tRNA pseudouridine 55 synthase; coiled-coil domain containing 139; coiled-coil domain-containing protein 139
• UniProt Protein Name: Putative tRNA pseudouridine synthase Pus10
• Protein Family: Putative tRNA pseudouridine synthase
• UniProt Gene Name: PUS10
• UniProt Synonym Gene Names: CCDC139; DOBI; Psi55 synthase
• UniProt Entry Name: PUS10_HUMAN