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Western Blot (WB) (NCOA7 Antibody (N-term) western blot analysis in Hela,MDA-MB-453 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the NCOA7 antibody detected the NCOA7 protein (arrow).)

Rabbit anti-Human, mouse NCOA7 Polyclonal Antibody | anti-NCOA7 antibody

NCOA7 Antibody (N-term)

Gene Names
NCOA7; ESNA1; TLDC4; ERAP140; NCOA7-AS; Nbla00052; Nbla10993; dJ187J11.3
Reactivity
Human, mouse
Applications
ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NCOA7; Polyclonal Antibody; NCOA7 Antibody (N-term); Nuclear receptor coactivator 7; 140 kDa estrogen receptor-associated protein; Estrogen nuclear receptor coactivator 1; ERAP140; ESNA1; anti-NCOA7 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NCOA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-103 amino acids from the N-terminal region of human NCOA7.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.35 (varies by lot)
Sequence Positions
76-103
Sequence Length
827
Applicable Applications for anti-NCOA7 antibody
ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(NCOA7 Antibody (N-term) western blot analysis in Hela,MDA-MB-453 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the NCOA7 antibody detected the NCOA7 protein (arrow).)

Western Blot (WB) (NCOA7 Antibody (N-term) western blot analysis in Hela,MDA-MB-453 cell line and mouse kidney tissue lysates (35ug/lane).This demonstrates the NCOA7 antibody detected the NCOA7 protein (arrow).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with NCOA7 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NCOA7 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (NCOA7 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-NCOA7 antibody
NCOA7 enhances the transcriptional activities of several nuclear receptors. It is involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA.
Product Categories/Family for anti-NCOA7 antibody
References
Arai, H., et al. Oncol. Rep. 19(6):1381-1388(2008)
?Liu, Y., et al. Schizophr. Res. 89 (1-3), 360-361 (2007) :
?Durand, M., et al. BMC Cell Biol. 8, 13 (2007) :
?Mungall, A.J., et al. Nature 425(6960):805-811(2003)
?Ohira, M., et al. Cancer Lett. 197 (1-2), 63-68 (2003) :
?Rushing, S.R., et al. Arch. Biochem. Biophys. 403(2):189-201(2002)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI GenBank Nucleotide #
Molecular Weight
106162
NCBI Official Full Name
nuclear receptor coactivator 7 isoform 2
NCBI Official Synonym Full Names
nuclear receptor coactivator 7
NCBI Official Symbol
NCOA7
NCBI Official Synonym Symbols
ESNA1; TLDC4; ERAP140; NCOA7-AS; Nbla00052; Nbla10993; dJ187J11.3
NCBI Protein Information
nuclear receptor coactivator 7
UniProt Protein Name
Nuclear receptor coactivator 7
UniProt Gene Name
NCOA7
UniProt Synonym Gene Names
ERAP140; ESNA1
UniProt Entry Name
NCOA7_HUMAN

Uniprot Description

NCOA7: Enhances the transcriptional activities of several nuclear receptors. Involved in the coactivation of different nuclear receptors, such as ESR1, THRB, PPARG and RARA. Belongs to the OXR1 family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q22.32

Cellular Component: intracellular; nucleus

Molecular Function: protein binding; ligand-dependent nuclear receptor transcription coactivator activity; nuclear hormone receptor binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter

Research Articles on NCOA7

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Product Notes

The NCOA7 ncoa7 (Catalog #AAA9215195) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 76-103. The NCOA7 Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NCOA7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the NCOA7 ncoa7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCOA7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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