Rabbit anti-Human PUS1 Polyclonal Antibody | anti-PUS1 antibody

PUS1 (Pseudouridylate Synthase 1, tRNA Pseudouridine Synthase A, Mitochondrial, tRNA Pseudouridine(38-40) Synthase, tRNA Pseudouridylate Synthase I, tRNA-uridine Isomerase I, MLASA1, PP8985)

Cat. Num. AAA6001533

• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PUS1; Polyclonal Antibody; PUS1 (Pseudouridylate Synthase 1; tRNA Pseudouridine Synthase A; Mitochondrial; tRNA Pseudouridine(38-40) Synthase; tRNA Pseudouridylate Synthase I; tRNA-uridine Isomerase I; MLASA1; PP8985); Anti -PUS1 (Pseudouridylate Synthase 1; anti-PUS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PUS1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MAGNAEPPPAGAACPQDRRSCSGRAGGDRVWEDGEHPAKKLKSGGDEERREKPPKRKIVLLMAYSGKGYHGMQRNVGSSQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDILEKINSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDRDVQDETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVREGLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVLERSWGTEKVDVPKAPGLGLVLERVHFEKYNQRFGNDGLHEPLDWAQEEGKVAAFKEEHIYPTIIGTERDERSMAQWLSTLPIHNFSATALTAGGTGAKVPSPLEGSEGDGDTD

• Applicable Applications for anti-PUS1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human PUS1, aa1-399 (AAH02901.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PUS1 expression in transfected 293T cell line by PUS1 polyclonal antibody. Lane 1: PUS1 transfected lysate (44kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-PUS1 antibody

Antibodies; Abs to Enzymes; Synthase

NCBI and Uniprot Product Information

• NCBI GI #: 6325044
• NCBI GeneID: 855889
• NCBI Accession #: NP_015112.1
• NCBI GenBank Nucleotide #: NM_001184026.1
• UniProt Accession #: Q12211; D6W3F8
• Molecular Weight: 62,143 Da
• NCBI Official Full Name: pseudouridine synthase PUS1
• NCBI Official Symbol: PUS1
• NCBI Protein Information: pseudouridine synthase PUS1
• UniProt Protein Name: tRNA pseudouridine synthase 1
• Protein Family: tRNA pseudouridine synthase
• UniProt Gene Name: PUS1
• UniProt Entry Name: PUS1_YEAST

Uniprot Description

Function: Formation of pseudouridine at positions 27 and 28 in the anticodon stem and loop of transfer RNAs; at positions 34 and 36 of intron-containing precursor tRNA(Ile) and at position 35 in the intron-containing tRNA(Tyr).

Catalytic activity: tRNA uridine = tRNA pseudouridine.

Cofactor: Binds 1 zinc ion per subunit.

Subcellular location: Nucleus Ref.5.

Miscellaneous: Present with 8130 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the tRNA pseudouridine synthase TruA family.