Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myosin-VIIa (MYO7A) Standard Curve.)
Mouse Myosin-VIIa (MYO7A) ELISA Kit | MYO7A elisa kit
Mouse Myosin-VIIa (MYO7A) ELISA Kit
Gene Names
Myo7a; Hdb; sh1; Myo7; sh-1; USH1B; polka; nmf371
Reactivity
Mouse
Synonyms
Myosin-VIIa (MYO7A); Mouse Myosin-VIIa (MYO7A) ELISA Kit; MYO7A; DFNA11; DFNB2; MYOVIIA; MYU7A; NSRD2; USH1B; deafness; autosomal dominant 11; autosomal recessive 2; myosin VIIA (Usher syndrome 1B (autosomal recessive; severe)); MYO7A elisa kit
Reactivity
Mouse
Specificity
Mouse Myosin-VIIa (MYO7A) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse MYO7A. No significant cross-reactivity or interference between Mouse MYO7A and analogues was observed.
Sequence Length
2215
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myosin-VIIa (MYO7A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Myosin-VIIa (MYO7A) Standard Curve.)
Related Product Information for MYO7A elisa kit
Description: This Mouse Myosin-VIIa (MYO7A) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO7A in samples. An antibody specific for MYO7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYO7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO7A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO7A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Myo7a is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain, an actin-binding domain, a neck domain that interacts with other proteins, and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness, reduced vestibular function, and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants.
Background: Myo7a is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain, an actin-binding domain, a neck domain that interacts with other proteins, and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness, reduced vestibular function, and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
unconventional myosin-VIIa isoform 1
NCBI Official Synonym Full Names
myosin VIIA
NCBI Official Symbol
Myo7a
NCBI Official Synonym Symbols
Hdb; sh1; Myo7; sh-1; USH1B; polka; nmf371
NCBI Protein Information
unconventional myosin-VIIa
UniProt Protein Name
Unconventional myosin-VIIa
Protein Family
UniProt Gene Name
Myo7a
UniProt Synonym Gene Names
Myo7
UniProt Entry Name
MYO7A_MOUSE
Research Articles on MYO7A
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Product Notes
The Mouse MYO7A myo7a (Catalog #AAA9717471) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717471 ELISA Kit recognizes Mouse MYO7A. It is sometimes possible for the material contained within the vial of "Myosin-VIIa (MYO7A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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