Rabbit anti-Human PUR4 Polyclonal Antibody | anti-PUR4 antibody

PUR4, NT (PFAS, KIAA0361, Phosphoribosylformylglycinamidine synthase, Formylglycinamide ribotide amidotransferase, Formylglycinamide ribotide synthetase)

Cat. Num. AAA648178

• Gene Names: PUR4; F1O17.7; F1O17_7; PUR4; purine biosynthesis 4
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PUR4; Polyclonal Antibody; NT (PFAS; KIAA0361; Phosphoribosylformylglycinamidine synthase; Formylglycinamide ribotide amidotransferase; Formylglycinamide ribotide synthetase); Anti -PUR4; anti-PUR4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PUR4 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: PUR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191~221 amino acids from the N-terminal region of human PUR4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PUR4 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). PUR4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PUR4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PUR4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PUR4 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PUR4 antibody

Antibodies; Abs to KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 186495325
• NCBI GeneID: 843766
• NCBI Accession #: NP_177566.3
• NCBI GenBank Nucleotide #: NM_106086.4
• UniProt Accession #: Q9M8D3; A9Y5J1; F4HTW2
• Molecular Weight: 153,954 Da
• NCBI Official Full Name: phosphoribosylformylglycinamidine synthase
• NCBI Official Symbol: PUR4
• NCBI Official Synonym Symbols: F1O17.7; F1O17_7; PUR4; purine biosynthesis 4
• NCBI Protein Information: phosphoribosylformylglycinamidine synthase
• UniProt Protein Name: Probable phosphoribosylformylglycinamidine synthase, chloroplastic/mitochondrial
• UniProt Gene Name: FGAM synthase
• UniProt Synonym Gene Names: FGAMS; FGARAT
• UniProt Entry Name: PUR4_ARATH

NCBI Description

Encodes formylglycinamidine ribonucleotide synthase an enzyme involved in de novo purine biosynthesis. PUR4 is localizes to the chloroplast and mitochondria. Loss of PUR4 function affects male but not female gametophyte development.

Uniprot Description

Function: Essential to the male gametophyte development. Ref.1

Catalytic activity: ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate.

Pathway: Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.

Subcellular location: Plastid › chloroplast. Mitochondrion Ref.1.

Disruption phenotype: Lethal to the male gametophyte. Ref.1

Sequence similarities: In the N-terminal section; belongs to the FGAMS family.Contains 1 glutamine amidotransferase type-1 domain.

Sequence caution: The sequence AAG52403.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence ABW87767.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.