Rabbit anti-Human PUR4 Polyclonal Antibody | anti-PUR4 antibody

PUR4, NT (PFAS, KIAA0361, Phosphoribosylformylglycinamidine synthase, Formylglycinamide ribotide amidotransferase, Formylglycinamide ribotide synthetase) (FITC)

Cat. Num. AAA6339165

• Gene Names: PFAS; PURL; FGAMS; GATD8; FGARAT; FGAR-AT
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PUR4; Polyclonal Antibody; NT (PFAS; KIAA0361; Phosphoribosylformylglycinamidine synthase; Formylglycinamide ribotide amidotransferase; Formylglycinamide ribotide synthetase) (FITC); PFAS; Formylglycinamide ribotide synthetase; anti-PUR4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PUR4 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PUR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191~221 amino acids from the N-terminal region of human PUR4.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PUR4 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). PUR4 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(PUR4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PUR4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PUR4 Antibody (N-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-PUR4 antibody

Antibodies; KIAA; orf

NCBI and Uniprot Product Information

• NCBI GI #: 31657129
• NCBI GeneID: 5198
• NCBI Accession #: NP_036525.1
• NCBI GenBank Nucleotide #: NM_012393.2
• UniProt Accession #: O15067; A6H8V8
• Molecular Weight: 144,734 Da
• NCBI Official Full Name: phosphoribosylformylglycinamidine synthase
• NCBI Official Synonym Full Names: phosphoribosylformylglycinamidine synthase
• NCBI Official Symbol: PFAS
• NCBI Official Synonym Symbols: PURL; FGAMS; GATD8; FGARAT; FGAR-AT
• NCBI Protein Information: phosphoribosylformylglycinamidine synthase
• UniProt Protein Name: Phosphoribosylformylglycinamidine synthase
• UniProt Gene Name: PFAS
• UniProt Synonym Gene Names: KIAA0361; FGAM synthase; FGAMS; FGAR amidotransferase; FGAR-AT

NCBI Description

Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. The enzyme encoded by this gene catalyzes the fourth step of IMP biosynthesis. [provided by RefSeq, Jul 2008]

Uniprot Description

PFAS: Phosphoribosylformylglycinamidine synthase involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate

Protein type: EC 6.3.5.3; Ligase; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: cytoplasm; cytosol

Molecular Function: ATP binding; metal ion binding; phosphoribosylformylglycinamidine synthase activity

Biological Process: de novo'IMP biosynthetic process; glutamine metabolic process; purine ribonucleoside monophosphate biosynthetic process; response to drug