Rabbit anti-Human PUM2 Polyclonal Antibody | anti-PUM2 antibody

PUM2, NT (Pumilio 2, Pumilio Homolog 2, Pumilio-2, KIAA0235, PUMH2)

Cat. Num. AAA629122

• Gene Names: PUM2; PUMH2; PUML2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: PUM2; Polyclonal Antibody; NT (Pumilio 2; Pumilio Homolog 2; Pumilio-2; KIAA0235; PUMH2); Anti -PUM2; anti-PUM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PUM2.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PUM2 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:50-1:100; Immunohistochemistry: 1:10-1:50
• Immunogen: Synthetic peptide selected from the N-terminal region of human PUM2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PUM2 (arrow) using rabbit polyclonal PUM2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PUM2 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with PUM2 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-PUM2 antibody

PUM2 is a member of the PUF family, evolutionarily conserved RNA-binding proteins related to the Pumilio proteins of Drosophila and the fem-3 mRNA binding factor proteins of C. elegans. This protein contains a sequence-specific RNA binding domain comprised of eight repeats and N- and C-terminal flanking regions, and serves as a translational regulator of specific mRNAs by binding to their 3' untranslated regions. The evolutionarily conserved function of this protein in invertebrates and lower vertebrates suggests that the human protein may be involved in translational regulation of embryogenesis, and cell development and differentiation.

Product Categories/Family for anti-PUM2 antibody

Antibodies; Abs to Stem Cell Research

NCBI and Uniprot Product Information

• NCBI GI #: 544583552
• NCBI GeneID: 23369
• NCBI Accession #: NP_001269720.1
• NCBI GenBank Nucleotide #: NM_001282791.1
• UniProt Accession #: Q8TB72; O00234; Q53TV7; Q8WY43; Q9HAN2; B3KSL0; D6W527
• Molecular Weight: 114,216 Da
• NCBI Official Full Name: pumilio homolog 2 isoform 4
• NCBI Official Synonym Full Names: pumilio RNA-binding family member 2
• NCBI Official Symbol: PUM2
• NCBI Official Synonym Symbols: PUMH2; PUML2
• NCBI Protein Information: pumilio homolog 2; pumilio-2
• UniProt Protein Name: Pumilio homolog 2
• Protein Family: Pumilio
• UniProt Gene Name: PUM2
• UniProt Synonym Gene Names: KIAA0235; PUMH2; Pumilio-2
• UniProt Entry Name: PUM2_HUMAN

NCBI Description

This gene encodes a protein that belongs to a family of RNA-binding proteins. The encoded protein functions as a translational repressor during embryonic development and cell differentiation. This protein is also thought to be a positive regulator of cell proliferation in adipose-derived stem cells. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

Pumilio-2: Sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Translation; RNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: nuclear membrane; perinuclear region of cytoplasm; stress granule; cytoplasm

Molecular Function: protein binding

Biological Process: regulation of translation; stress granule assembly