Western Blot (WB)
(PUF60 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PUF60 antibody detected the PUF60 protein (arrow).)
Rabbit anti-Human PUF60 Polyclonal Antibody | anti-PUF60 antibody
PUF60 Antibody (N-term)
Western Blot (WB)
(PUF60 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PUF60 antibody detected the PUF60 protein (arrow).)
Western Blot (WB)
(PUF60 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PUF60 antibody detected the PUF60 protein (arrow).)
protein. It interacts with Ro RNPs and their interaction is thought
to represent a gain of function for Ro RNPs. This protein also
forms a ternary complex with far upstream element (FUSE) and
FUSE-binding protein. It can repress a c-myc reporter via the FUSE.
It is also known to target transcription factor IIH and inhibit
activated transcription. This gene is implicated in the xeroderma
pigmentosum disorder. There are two alternatively spliced
transcript variants of this gene encoding different isoforms. There
seems to be evidence of multiple polyadenylation sites for this
gene.
Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009)
Gao, J., et al. Genomics 91(4):347-355(2008)
Hastings, M.L., et al. PLoS ONE 2 (6), E538 (2007) :
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
Uniprot Description
FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.
Protein type: Transcription, coactivator/corepressor; RNA-binding; RNA splicing; Spliceosome
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; ribonucleoprotein complex; cyclin-dependent protein kinase activating kinase holoenzyme complex
Molecular Function: identical protein binding; protein binding; DNA binding; nucleotide binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; RNA splicing; mRNA processing
Disease: Verheij Syndrome
Research Articles on PUF60
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Product Notes
The PUF60 puf60 (Catalog #AAA9208219) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 15-43. The PUF60 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PUF60 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the PUF60 puf60 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PUF60, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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