Rabbit anti-Human PUF60 Polyclonal Antibody | anti-PUF60 antibody

PUF60, NT (PUF60, FIR, ROBPI, SIAHBP1, Poly(U)-binding-splicing factor PUF60, 60kD poly(U)-binding-splicing factor, FUSE-binding protein-interacting repressor, Ro-binding protein 1, Siah-binding protein 1) (PE)

Cat. Num. AAA6339161

• Gene Names: PUF60; FIR; VRJS; RoBPI; SIAHBP1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PUF60; Polyclonal Antibody; NT (PUF60; FIR; ROBPI; SIAHBP1; Poly(U)-binding-splicing factor PUF60; 60kD poly(U)-binding-splicing factor; FUSE-binding protein-interacting repressor; Ro-binding protein 1; Siah-binding protein 1) (PE); Siah-binding protein 1; anti-PUF60 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-PUF60 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PUF60 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-43 amino acids from the N-terminal region of human PUF60.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PUF60 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the PUF60 antibody detected the PUF60 protein (arrow).)

Related Product Information for anti-PUF60 antibody

The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.

Product Categories/Family for anti-PUF60 antibody

Antibodies; Splicing Factors

References

Hsiao, H.H., et al. Biochemistry 49(22):4620-4634(2010). Corsini, L., et al. J. Biol. Chem. 284(1):630-639(2009). Gao, J., et al. Genomics 91(4):347-355(2008). Hastings, M.L., et al. PLoS ONE 2 (6), E538 (2007): Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 17978512
• NCBI GeneID: 22827
• NCBI Accession #: NP_510965.1; NP_055096.2; NP_001129505.1
• NCBI GenBank Nucleotide #: NM_078480.2
• UniProt Accession #: Q9UHX1; Q969E7; Q96D94; Q96H63; Q99628; Q9NZA0; Q9UJY7; A8K8K8
• Molecular Weight: 55,399 Da
• NCBI Official Full Name: poly(U)-binding-splicing factor PUF60 isoform a
• NCBI Official Synonym Full Names: poly(U) binding splicing factor 60KDa
• NCBI Official Symbol: PUF60
• NCBI Official Synonym Symbols: FIR; VRJS; RoBPI; SIAHBP1
• NCBI Protein Information: poly(U)-binding-splicing factor PUF60
• UniProt Protein Name: Poly(U)-binding-splicing factor PUF60
• Protein Family: Poly(U)-binding-splicing factor
• UniProt Gene Name: PUF60
• UniProt Synonym Gene Names: FIR; ROBPI; SIAHBP1; FBP-interacting repressor; RoBP1; Siah-BP1
• UniProt Entry Name: PUF60_HUMAN

NCBI Description

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

FIR: fuse-binding protein-interacting repressor. Interacts with (and may activate) Ro RNPs. Forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. Two alternatively spliced isoforms have been described.

Protein type: RNA splicing; RNA-binding; Spliceosome; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: nucleoplasm; Golgi apparatus; mitochondrion; ribonucleoprotein complex; cyclin-dependent protein kinase activating kinase holoenzyme complex

Molecular Function: identical protein binding; protein binding; DNA binding; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; RNA splicing; mRNA processing

Disease: Verheij Syndrome