Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PTPN13 antibody at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.)
Rabbit PTPN13 Polyclonal Antibody | anti-PTPN13 antibody
PTPN13 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
PTPN13; Polyclonal Antibody; PTPN13 Polyclonal Antibody; FAP-1; PNP1; PTP-BAS; PTP-BL; PTP1E; PTPL1; PTPLE; hPTP1E; anti-PTPN13 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 248% glycerol.
PBS with 0.02% sodium azide, 50% glycerol, pH7.201
PBS with 0.02% sodium azide, 50% glycerol, pH7.201
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PTPN13 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IF: 1:50-1:100
IHC: 1:50-1:100
IF: 1:50-1:100
Immunogen
Recombinant fusion protein of human PTPN13 (NP_542416.1)
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PTPN13 antibody at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PTPN13 antibody at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH-3T3 cells using PTPN13 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH-3T3 cells using PTPN13 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)
Related Product Information for anti-PTPN13 antibody
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large intracellular protein. It has a catalytic PTP domain at its C-terminus and two major structural domains: a region with five PDZ domains and a FERM domain that binds to plasma membrane and cytoskeletal elements. This PTP was found to interact with, and dephosphorylate, Fas receptor and IkappaBalpha through the PDZ domains. This suggests it has a role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathways. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported.
Product Categories/Family for anti-PTPN13 antibody
NCBI and Uniprot Product Information
NCBI GeneID
UniProt Accession #
Molecular Weight
MW: 255kDa, 274kDa, 276kDa, 277kDa
SDS PAGE MW: 310kDa
SDS PAGE MW: 310kDa
UniProt Protein Name
Tyrosine-protein phosphatase non-receptor type 13
Protein Family
UniProt Gene Name
PTPN13
UniProt Synonym Gene Names
PNP1; PTP1E; PTPL1; FAP-1; PTP-E1; hPTPE1
UniProt Entry Name
PTN13_HUMAN
Uniprot Description
PTPN13: Tyrosine phosphatase which regulates negatively FAS- induced apoptosis and NGFR-mediated pro-apoptotic signaling. Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)
Chromosomal Location of Human Ortholog: 4q21.3
Cellular Component: neuron projection; cytoskeleton; lamellipodium; cytoplasm; plasma membrane; nucleus
Molecular Function: protein binding; protein tyrosine phosphatase activity
Biological Process: regulation of phosphoinositide 3-kinase cascade; protein amino acid dephosphorylation
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Product Notes
The PTPN13 ptpn13 (Catalog #AAA9457643) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTPN13 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PTPN13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:100 IF: 1:50-1:100. Researchers should empirically determine the suitability of the PTPN13 ptpn13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPN13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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