Western Blot (WB)
(PTPLAD1 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PTPLAD1 antibody detected the PTPLAD1 protein (arrow).)
Rabbit anti-Human PTPLAD1 Polyclonal Antibody | anti-PTPLAD1 antibody
PTPLAD1, NT (PTPLAD1, BIND1, HACD3, 3-hydroxyacyl-CoA dehydratase 3, Butyrate-induced protein 1, Protein tyrosine phosphatase-like protein PTPLAD1, Protein-tyrosine phosphatase-like A domain-containing protein 1) (AP)
Gene Names
PTPLAD1; BIND1; HACD3; B-IND1; HSPC121
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
PTPLAD1; Polyclonal Antibody; NT (PTPLAD1; BIND1; HACD3; 3-hydroxyacyl-CoA dehydratase 3; Butyrate-induced protein 1; Protein tyrosine phosphatase-like protein PTPLAD1; Protein-tyrosine phosphatase-like A domain-containing protein 1) (AP); Protein-tyrosine phosphatase-like A domain-containing protein 1; anti-PTPLAD1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-PTPLAD1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
PTPLAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 31-58 amino acids selected from the N-terminal region of human PTPLAD1.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(PTPLAD1 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PTPLAD1 antibody detected the PTPLAD1 protein (arrow).)
Western Blot (WB)
(PTPLAD1 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the PTPLAD1 antibody detected the PTPLAD1 protein (arrow).)
Related Product Information for anti-PTPLAD1 antibody
Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis. Involved in Rac1-signaling pathways leading to the modulation of gene expression.
Product Categories/Family for anti-PTPLAD1 antibody
References
Kamatani, Y., et al. Nat. Genet. 42(3):210-215(2010). Taguwa, S., et al. J. Virol. 83(20):10427-10436(2009). Taguwa, S., et al. J. Virol. 82(6):2631-2641(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Sabbah, M., et al. Gene 374, 128-133 (2006).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,160 Da
NCBI Official Full Name
very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3
NCBI Official Synonym Full Names
protein tyrosine phosphatase-like A domain containing 1
NCBI Official Symbol
PTPLAD1
NCBI Official Synonym Symbols
BIND1; HACD3; B-IND1; HSPC121
NCBI Protein Information
very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3; very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3; hB-ind1; butyrate-induced protein 1; butyrate-induced transcript 1; protein tyrosine phosphatase-like protein PTPLAD1; protein-tyrosine phosphatase
UniProt Protein Name
Very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 3
UniProt Gene Name
PTPLAD1
UniProt Synonym Gene Names
BIND1; HACD3; HACD3; B-ind1; hB-ind1
UniProt Entry Name
HACD3_HUMAN
Uniprot Description
Function: Responsible for the dehydration step in very long-chain fatty acid (VLCFA) synthesis. Involved in Rac1-signaling pathways leading to the modulation of gene expression. Ref.1 Ref.8
Catalytic activity: A very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] = a very-long-chain trans-2,3-dehydroacyl-[acyl-carrier protein] + H2O. Ref.8
Subunit structure: Interacts with the condensation enzymes of the ELOVL family. Interacts with RAC1. Ref.1 Ref.8
Subcellular location: Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.8.
Tissue specificity: Highly expressed in testis, kidney, brain, liver and weakly in skeletal muscle, spleen and heart. No expression detected in leukocytes. Ref.1 Ref.8
Induction: By AKAP12 and histone deacetylase inhibitors such as sodium butyrate. Ref.6
Sequence similarities: Belongs to the very long-chain fatty acids dehydratase HACD family.Contains 1 CS domain.
Sequence caution: The sequence AAF29085.1 differs from that shown. Reason: Frameshift at position 356. The sequence BAC11249.1 differs from that shown. Reason: Frameshift at position 359.
Similar Products
Product Notes
The PTPLAD1 ptplad1 (Catalog #AAA6338920) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTPLAD1, NT (PTPLAD1, BIND1, HACD3, 3-hydroxyacyl-CoA dehydratase 3, Butyrate-induced protein 1, Protein tyrosine phosphatase-like protein PTPLAD1, Protein-tyrosine phosphatase-like A domain-containing protein 1) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PTPLAD1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PTPLAD1 ptplad1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTPLAD1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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