Rabbit anti-Human PTPLA Polyclonal Antibody | anti-PTPLA antibody

PTPLA, NT (PTPLA, HACD1, 3-hydroxyacyl-CoA dehydratase 1, Cementum attachment protein, Protein-tyrosine phosphatase-like member A) (AP)

Cat. Num. AAA6338909

• Gene Names: PTPLA; CAP; HACD1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTPLA; Polyclonal Antibody; NT (PTPLA; HACD1; 3-hydroxyacyl-CoA dehydratase 1; Cementum attachment protein; Protein-tyrosine phosphatase-like member A) (AP); Protein-tyrosine phosphatase-like member A; anti-PTPLA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-PTPLA antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PTPLA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids from the N-terminal region of human PTPLA.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PTPLA Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the PTPLA antibody detected the PTPLA protein (arrow).)

Immunohistochemistry (IHC)

(PTPLA Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTPLA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PTPLA antibody

The protein encoded by this gene contains a characteristic catalytic motif of the protein tyrosine phosphatases (PTPs) family. The PTP motif of this protein has the highly conserved arginine residue replaced by a proline residue; thus it may represent a distinct class of PTPs. Members of the PTP family are known to be signaling molecules that regulate a variety of cellular processes. This gene was preferentially expressed in both adult and fetal heart. A much lower expression level was detected in skeletal and smooth muscle tissues, and no expression was observed in other tissues. The tissue specific expression in the developing and adult heart suggests a role in regulating cardiac development and differentiation.

Product Categories/Family for anti-PTPLA antibody

Antibodies; Enzymes; Phosphatase

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Liang, X., et al. Hum. Mutat. 30(3):463-471(2009). Lamesch, P., et al. Genomics 89(3):307-315(2007). Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 82659105
• NCBI GeneID: 9200
• NCBI Accession #: NP_055056.3
• NCBI GenBank Nucleotide #: NM_014241.3
• UniProt Accession #: B0YJ81; Q6JIC5; Q96FW7; Q9HB93; Q9UHX2; B0YJ80
• Molecular Weight: 14,920 Da
• NCBI Official Full Name: very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase-like (proline instead of catalytic arginine), member A
• NCBI Official Symbol: PTPLA
• NCBI Official Synonym Symbols: CAP; HACD1
• NCBI Protein Information: very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1; very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1; cementum attachment protein; very-long-chain (3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 1
• UniProt Protein Name: Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1
• UniProt Gene Name: PTPLA
• UniProt Synonym Gene Names: HACD1; HACD1
• UniProt Entry Name: HACD1_HUMAN

NCBI Description

The protein encoded by this gene contains a characteristic catalytic motif of the protein tyrosine phosphatases (PTPs) family. The PTP motif of this protein has the highly conserved arginine residue replaced by a proline residue; thus it may represent a distinct class of PTPs. Members of the PTP family are known to be signaling molecules that regulate a variety of cellular processes. This gene was preferentially expressed in both adult and fetal heart. A much lower expression level was detected in skeletal and smooth muscle tissues, and no expression was observed in other tissues. The tissue specific expression in the developing and adult heart suggests a role in regulating cardiac development and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

PTPLA: Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis. Belongs to the very long-chain fatty acids dehydratase HACD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; EC 4.2.1.134; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p12.33

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: enzyme binding; hydroxyapatite binding; lyase activity; 3-hydroxyacyl-CoA dehydratase activity

Biological Process: sphingolipid biosynthetic process; multicellular organismal development; regulation of G2/M transition of mitotic cell cycle; very-long-chain fatty acid biosynthetic process; myotube differentiation; fatty acid elongation; fatty acid biosynthetic process