Human 3-Hydroxyacyl-CoA Dehydratase 1 (PTPLA) ELISA Kit | PTPLA elisa kit

Human 3-Hydroxyacyl-CoA Dehydratase 1 (PTPLA) ELISA Kit

Cat. Num. AAA9714718

• Gene Names: HACD1; CAP; PTPLA
• Reactivity: Human
• Synonyms: 3-Hydroxyacyl-CoA Dehydratase 1 (PTPLA); Human 3-Hydroxyacyl-CoA Dehydratase 1 (PTPLA) ELISA Kit; PTPLA; CAP; cementum attachment protein; protein tyrosine phosphatase-like; member A; protein-tyrosine phosphatase-like member A; PTPLA elisa kit

• Reactivity: Human
• Sequence Length: 288

• Assay Type: Sandwich
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTPLA elisa kit

Principle of the Assay: Human 3-Hydroxyacyl-CoA Dehydratase 1 (PTPLA) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPLA in samples. An antibody specific for PTPLA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTPLA present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human PTPLA detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of PTPLA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background/Introduction: Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.

NCBI and Uniprot Product Information

• NCBI GI #: 82659105
• NCBI GeneID: 9200
• NCBI Accession #: NP_055056.3
• NCBI GenBank Nucleotide #: NP_055056.3
• UniProt Accession #: B0YJ81
• NCBI Official Full Name: very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1
• NCBI Official Synonym Full Names: 3-hydroxyacyl-CoA dehydratase 1
• NCBI Official Symbol: HACD1
• NCBI Official Synonym Symbols: CAP; PTPLA
• NCBI Protein Information: very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1
• UniProt Protein Name: Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 1
• UniProt Gene Name: PTPLA
• UniProt Synonym Gene Names: HACD1; HACD1
• UniProt Entry Name: HACD1_HUMAN

NCBI Description

The protein encoded by this gene contains a characteristic catalytic motif of the protein tyrosine phosphatases (PTPs) family. The PTP motif of this protein has the highly conserved arginine residue replaced by a proline residue; thus it may represent a distinct class of PTPs. Members of the PTP family are known to be signaling molecules that regulate a variety of cellular processes. This gene was preferentially expressed in both adult and fetal heart. A much lower expression level was detected in skeletal and smooth muscle tissues, and no expression was observed in other tissues. The tissue specific expression in the developing and adult heart suggests a role in regulating cardiac development and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

PTPLA: Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis. Belongs to the very long-chain fatty acids dehydratase HACD family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; EC 4.2.1.134; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10p12.33

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; integral to membrane

Molecular Function: enzyme binding; hydroxyapatite binding; lyase activity; 3-hydroxyacyl-CoA dehydratase activity

Biological Process: sphingolipid biosynthetic process; multicellular organismal development; regulation of G2/M transition of mitotic cell cycle; very-long-chain fatty acid biosynthetic process; myotube differentiation; fatty acid elongation; fatty acid biosynthetic process