Rabbit anti-Human EP3 Polyclonal Antibody | anti-EP3 antibody

EP3 (R39) Polyclonal Antibody

Cat. Num. AAA3003493

• Gene Names: PTGER3; EP3; EP3e; EP3-I; EP3-II; EP3-IV; EP3-VI; PGE2-R; EP3-III; lnc003875
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: EP3; Polyclonal Antibody; EP3 (R39) Polyclonal Antibody; Prostaglandin E2 receptor EP3 subtype; PGE receptor EP3 subtype; PGE2 receptor EP3 subtype; PGE2-R; Prostanoid EP3 receptor; PTGER3; anti-EP3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: EP3 (R39) polyclonal antibody detects endogenous levels of EP3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 390

• Applicable Applications for anti-EP3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human EP3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of EP3 (R39) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-EP3 antibody

Prostaglandin E2 binds to four G protein-coupled E-prostanoid receptors, designated EP1, EP2, EP3 and EP4. The expression and function of the prostaglandin E2 receptors have been highly characterized in kidney. EP3, which is expressed in renal vessels, thick ascending limb and collecting duct, has at least six alternative splice variants that couple to Gi proteins to inhibit cAMP, which subsequently inhibit sodium and water transport. In uterus, EP3 induces the contraction of uterine smooth muscles. EP4 is expressed in glomerulus and collecting duct.

NCBI and Uniprot Product Information

• NCBI GI #: 186659506
• NCBI GeneID: 5733
• NCBI Accession #: NP_001119516.1
• NCBI GenBank Nucleotide #: NP_001119516.1
• UniProt Accession #: P43115
• Molecular Weight: ~ 40kDa
• NCBI Official Full Name: prostaglandin E2 receptor EP3 subtype isoform 4
• NCBI Official Synonym Full Names: prostaglandin E receptor 3
• NCBI Official Symbol: PTGER3
• NCBI Official Synonym Symbols: EP3; EP3e; EP3-I; EP3-II; EP3-IV; EP3-VI; PGE2-R; EP3-III; lnc003875
• NCBI Protein Information: prostaglandin E2 receptor EP3 subtype
• UniProt Protein Name: Prostaglandin E2 receptor EP3 subtype
• Protein Family: Endochitinase
• UniProt Gene Name: PTGER3
• UniProt Synonym Gene Names: PGE receptor EP3 subtype
• UniProt Entry Name: PE2R3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor may have many biological functions, which involve digestion, nervous system, kidney reabsorption, and uterine contraction activities. Studies of the mouse counterpart suggest that this receptor may also mediate adrenocorticotropic hormone response as well as fever generation in response to exogenous and endogenous stimuli. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2009]

Uniprot Description

PTGER3: Receptor for prostaglandin E2 (PGE2); the EP3 receptor may be involved in inhibition of gastric acid secretion, modulation of neurotransmitter release in central and peripheral neurons, inhibition of sodium and water reabsorption in kidney tubulus and contraction in uterine smooth muscle. The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G-I proteins, and to an elevation of intracellular calcium. The various isoforms have identical ligand binding properties but can interact with different second messenger systems. Belongs to the G-protein coupled receptor 1 family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 1p31.2

Cellular Component: integral to plasma membrane; integral to membrane; plasma membrane; nuclear envelope

Molecular Function: ligand-dependent nuclear receptor activity; prostaglandin E receptor activity

Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; cell death; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; bicarbonate transport; positive regulation of fever; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); response to lipopolysaccharide; fever