Goat PTF1A/PFT1-P48 Polyclonal Antibody | anti-PTF1A antibody

Goat anti-PTF1A/PFT1-P48 Antibody

Cat. Num. AAA420223

• Gene Names: PTF1A; PACA; PAGEN2; bHLHa29; PTF1-p48
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Zebrafish
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: PTF1A/PFT1-P48; Polyclonal Antibody; Goat anti-PTF1A/PFT1-P48 Antibody; PTF1A; PTF1-p48; pancreas specific transcription factor; 1a; bHLH transcription factor p48; class II bHLH protein PTF1A; exocrine pancreas-specific transcription factor p48; p48 DNA-binding subunit of transcription factor PTF1; pancreas transcription fact; PTF1A antibody; PTF1-p48 antibody; 1a antibody; bHLH transcription factor p48 antibody; class II bHLH protein PTF1A antibody; exocrine pancreas-specific transcription factor p48 antibody; p48 DNA-binding subunit of transcription factor PTF1 antibody; pancreas transcription factor 1 subunit alpha antibody; PTF1A / PFT1-P48; anti-PTF1A antibody

• Host: Goat
• Reactivity: Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Zebrafish
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: WTDEKQLKEQN
• Sequence Length: 328

• Applicable Applications for anti-PTF1A antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:128000.; WB: Approx 37kDa observe in Human Pancreas lysates and approx. 38-40kDa in lysates of cell lines Jurkat, U2OS and NIH3T3 , (calculated MW of 35.0kDa according to Human NP_835455.1 and 35.2kDa according to Mouse NP_061279.2). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature.
• Immunogen: Peptide with sequence C-WTDEKQLKEQN, from the internal region of the protein sequence according to NP_835455.1.
• Epitope: Internal region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Testing Data

(MBS420223 (1µg/ml) staining of U2OS (A) + peptide (B), (0.5ug/ml) Jurkat (C) + peptide (D) and NIH3T3 (E) + peptide (F) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)

Western Blot

(MBS420223 (0.5µg/ml) staining of Human Pancreas lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 30039710
• NCBI GeneID: 256297
• NCBI Accession #: NP_835455.1
• NCBI GenBank Nucleotide #: NM_178161.2
• NCBI Official Full Name: pancreas transcription factor 1 subunit alpha
• NCBI Official Synonym Full Names: pancreas specific transcription factor, 1a
• NCBI Official Symbol: PTF1A
• NCBI Official Synonym Symbols: PACA; PAGEN2; bHLHa29; PTF1-p48
• NCBI Protein Information: pancreas transcription factor 1 subunit alpha
• UniProt Protein Name: Pancreas transcription factor 1 subunit alpha
• Protein Family: Pancreas transcription factor
• UniProt Gene Name: PTF1A
• UniProt Synonym Gene Names: BHLHA29; PTF1P48; bHLHa29; PTF1-p48
• UniProt Entry Name: PTF1A_HUMAN

NCBI Description

This gene encodes a protein that is a component of the pancreas transcription factor 1 complex (PTF1) and is known to have a role in mammalian pancreatic development. The protein plays a role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. The protein is thought to be involved in the maintenance of exocrine pancreas-specific gene expression including elastase 1 and amylase. Mutations in this gene cause cerebellar agenesis and loss of expression is seen in ductal type pancreas cancers. [provided by RefSeq, Jul 2008]

Uniprot Description

PTF1A: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH).

Chromosomal Location of Human Ortholog: 10p12.2

Cellular Component: cytoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; DNA binding; protein dimerization activity

Biological Process: cerebellum development; embryonic development; exocrine pancreas development; neuron fate commitment; pancreas development; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; retinoic acid receptor signaling pathway; tissue development; transcription from RNA polymerase II promoter

Disease: Pancreatic Agenesis 2; Pancreatic And Cerebellar Agenesis