Rabbit anti-Human PTDSS2 Polyclonal Antibody | anti-PTDSS2 antibody

PTDSS2, NT (PTDSS2, PSS2, Phosphatidylserine synthase 2, Serine-exchange enzyme II) (FITC)

Cat. Num. AAA6338483

• Gene Names: PTDSS2; PSS2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTDSS2; Polyclonal Antibody; NT (PTDSS2; PSS2; Phosphatidylserine synthase 2; Serine-exchange enzyme II) (FITC); Serine-exchange enzyme II; anti-PTDSS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PTDSS2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PTDSS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PTDSS2.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PTDSS2 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the PTDSS2 antibody detected the PTDSS2 protein (arrow).)

Immunohistochemistry (IHC)

(PTDSS2 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTDSS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PTDSS2 antibody

Phosphatidylserine (PS) accounts for 5 to 10% of cell membrane phospholipids. In addition to its role as a structural component, PS is involved in cell signaling, blood coagulation, and apoptosis. PS is synthesized by a calcium-dependent base-exchange reaction catalyzed by PS synthases (EC 2.7.8.8), like PTDSS2, that exchange L-serine for the polar head group of phosphatidylcholine (PC) or phosphatidylethanolamine (PE) (Sturbois-Balcerzak et al., 2001 [PubMed 11084049]).

Product Categories/Family for anti-PTDSS2 antibody

Antibodies; Enzymes; Synthase

References

Tomohiro, S., et al. Biochem. J. 418(2):421-429(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006). Olsen, J.V., et al. Cell 127(3):635-648(2006). Grandmaison, P.A., et al. Biochim. Biophys. Acta 1636(1):1-11(2004). Sturbois-Balcerzak, B., et al. J. Biol. Chem. 276(11):8205-8212(2001).

NCBI and Uniprot Product Information

• NCBI GI #: 13540555
• NCBI GeneID: 81490
• NCBI Accession #: NP_110410.1
• NCBI GenBank Nucleotide #: NM_030783.2
• UniProt Accession #: Q9BVG9
• Molecular Weight: 56,253 Da
• NCBI Official Full Name: phosphatidylserine synthase 2 isoform 2
• NCBI Official Synonym Full Names: phosphatidylserine synthase 2
• NCBI Official Symbol: PTDSS2
• NCBI Official Synonym Symbols: PSS2
• NCBI Protein Information: phosphatidylserine synthase 2
• UniProt Protein Name: Phosphatidylserine synthase 2
• Protein Family: Phosphatidylserine synthase
• UniProt Gene Name: PTDSS2
• UniProt Synonym Gene Names: PSS2; PSS-2; PtdSer synthase 2

NCBI Description

The protein encoded by this gene catalyzes the conversion of phosphatidylethanolamine to phosphatidylserine, a structural membrane phospholipid that functions in cell signaling, blood coagulation, and apoptosis. The encoded enzyme also has a high affinity for docosahexaenoic acid (DHA) and can use it to make DHA-containing phosphatidylserine. [provided by RefSeq, Jul 2016]

Uniprot Description

PTDSS2: Catalyzes a base-exchange reaction in which the polar head group of phosphatidylethanolamine (PE) or phosphatidylcholine (PC) is replaced by L-serine. PTDSS2 is specific for phosphatatidylethanolamine and does not act on phosphatidylcholine. Belongs to the phosphatidyl serine synthase family.

Protein type: EC 2.7.8.29; Lipid Metabolism - glycerophospholipid; Membrane protein, integral; Membrane protein, multi-pass; Transferase

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: endoplasmic reticulum membrane; integral component of membrane; membrane

Molecular Function: CDP-diacylglycerol-serine O-phosphatidyltransferase activity; transferase activity

Biological Process: phosphatidylserine biosynthetic process