Western Blot (WB)
((0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat PTBP1/PTB Polyclonal Antibody | anti-PTBP1 antibody
Goat anti-PTBP1/PTB Antibody
Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules. Recommended concentration, 2-4ug/ml.
Western Blot: Approx 55kDa band observed in Human Liver and Ovary lysates, and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and HEK293 (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 01ug/ml) staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.)
Immunohistochemistry (IHC)
((2ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi-RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
hnRNP I: a ubiquitously expressed heterogeneous nuclear ribonucleoprotein (hnRNP). hnRNPs are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Two alternatively spliced isoforms have been described.
Protein type: RNA splicing; RNA-binding
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: membrane; nucleolus; nucleoplasm
Molecular Function: nucleotide binding; poly-pyrimidine tract binding; protein binding; RNA binding
Biological Process: fibroblast growth factor receptor signaling pathway; gene expression; mRNA processing; negative regulation of muscle cell differentiation; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of RNA splicing; nuclear mRNA splicing, via spliceosome; regulation of alternative nuclear mRNA splicing, via spliceosome; RNA splicing
Research Articles on PTBP1
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Product Notes
The PTBP1 ptbp1 (Catalog #AAA420820) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PTBP1/PTB Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's PTBP1/PTB can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 32000. Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear membrane staining in all the convoluted tubules. Recommended concentration, 2-4ug/ml. Western Blot: Approx 55kDa band observed in Human Liver and Ovary lysates, and 60kDa band observed in nuclear lysates of cell lines HeLa, HepG2, Jurkat and HEK293 (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the PTBP1 ptbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DGIVPDIAVG TKR. It is sometimes possible for the material contained within the vial of "PTBP1/PTB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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