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Western Blot (WB)
(Western blot analysis of extracts of various cell lines using PTBP1 Polyclonal Antibody at dilution of 1:1000.)
Rabbit PTBP1 Polyclonal Antibody | anti-PTBP1 antibody
PTBP1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
PTBP1; Polyclonal Antibody; PTBP1 Polyclonal Antibody; HNRNP-I; HNRNPI; HNRPI; PTB; PTB-1; PTBT; PTB2; PTB3; PTB4; pPTB; anti-PTBP1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Sequence Length
177
Applicable Applications for anti-PTBP1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50-1:200
Note: Centrifuge before opening to ensure complete recovery of vial contents.
IHC: 1:50 - 1:200
IF: 1:50-1:200
Note: Centrifuge before opening to ensure complete recovery of vial contents.
Immunogen
A synthetic peptide of human PTBP1 (NP_114368.1).
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-16547 / sc-16549
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines using PTBP1 Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines using PTBP1 Polyclonal Antibody at dilution of 1:1000.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate cancer using PTBP1 antibody at dilution of1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate cancer using PTBP1 antibody at dilution of1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using PTBP1 antibody at dilution of1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using PTBP1 antibody at dilution of1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin embedded Human tonsil using PTBP1 Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin embedded Human tonsil using PTBP1 Polyclonal Antibody at dilution of 1:100 (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using PTBP1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using PTBP1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Related Product Information for anti-PTBP1 antibody
Background: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA-binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has four repeats of quasi- RNA recognition motif (RRM) domains that bind RNAs. This protein binds to the intronic polypyrimidine tracts that requires pre-mRNA splicing and acts via the protein degradation ubiquitin-proteasome pathway. It may also promote the binding of U2 snRNP to pre-mRNAs. This protein is localized in the nucleoplasm and it is also detected in the perinucleolar structure. Alternatively spliced transcript variants encoding different isoforms have been described.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
Calculated MW 57kDa/59kDa
Observed MW 57kDa
Observed MW 57kDa
NCBI Official Full Name
PTBP1, partial
Protein Family
Similar Products
Product Notes
The PTBP1 (Catalog #AAA2540752) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PTBP1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PTBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50-1:200 Note: Centrifuge before opening to ensure complete recovery of vial contents. Researchers should empirically determine the suitability of the PTBP1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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