Monkey Cytokine receptor common subunit gamma (IL2RG) ELISA Kit | IL2RG elisa kit
Monkey Cytokine receptor common subunit gamma (IL2RG) ELISA Kit
Principle of the Assay: IL2RG ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL2RG antibody and an IL2RG-HRP conjugate. The assay sample and buffer are incubated together with IL2RG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL2RG concentration since IL2RG from samples and IL2RG-HRP conjugate compete for the anti-IL2RG antibody binding site. Since the number of sites is limited, as more sites are occupied by IL2RG from the sample, fewer sites are left to bind IL2RG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL2RG concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in this gene cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder. [provided by RefSeq, Mar 2010]
Uniprot Description
IL2RG: Common subunit for the receptors for a variety of interleukins. Defects in IL2RG are the cause of severe combined immunodeficiency X-linked T-cell-negative/B-cell-positive/NK-cell- negative (XSCID); also known as agammaglobulinemia Swiss type. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell- mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Defects in IL2RG are the cause of X-linked combined immunodeficiency (XCID). XCID is a less severe form of X-linked immunodeficiency with a less severe degree of deficiency in cellular and humoral immunity than that seen in XSCID. Belongs to the type I cytokine receptor family. Type 5 subfamily.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: Xq13.1
Cellular Component: membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane
Molecular Function: protein binding; interleukin-4 receptor activity; interleukin-2 receptor activity; interleukin-7 receptor activity; interleukin-7 binding; interleukin-2 binding
Biological Process: viral reproduction; immune response; signal transduction
Disease: Combined Immunodeficiency, X-linked; Severe Combined Immunodeficiency, X-linked
Research Articles on IL2RG
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Product Notes
The Monkey IL2RG il2rg (Catalog #AAA7207256) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7207256 ELISA Kit recognizes Monkey IL2RG. It is sometimes possible for the material contained within the vial of "Cytokine receptor common subunit gamma (IL2RG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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