Rat Superoxide Dismutase, Copper Chaperone (SOD4) ELISA Kit | SOD4 elisa kit

Rat Superoxide Dismutase, Copper Chaperone (SOD4) ELISA Kit

Cat. Num. AAA2702113

• Gene Names: sod4; sod4ap; SODCC.2; GRMZM2G169890
• Reactivity: Rat
• Synonyms: Superoxide Dismutase; Copper Chaperone (SOD4); Rat Superoxide Dismutase; Copper Chaperone (SOD4) ELISA Kit; CCS; Copper Chaperone For Superoxide Dismutase; SOD4 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Superoxide Dismutase, Copper Chaperone (SOD4).; No significant cross-reactivity or interference between Superoxide Dismutase, Copper Chaperone (SOD4) and analogues was observed.
• Sequence Length: 152

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Superoxide Dismutase, Copper Chaperone (SOD4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Superoxide Dismutase, Copper Chaperone (SOD4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SOD4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Superoxide Dismutase, Copper Chaperone (SOD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Superoxide Dismutase, Copper Chaperone (SOD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Superoxide Dismutase, Copper Chaperone (SOD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Superoxide Dismutase, Copper Chaperone (SOD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 162463249
• NCBI GeneID: 542722
• NCBI Accession #: NP_001105704.1
• NCBI GenBank Nucleotide #: NM_001112234.1
• Molecular Weight: 15,071 Da
• NCBI Official Full Name: superoxide dismutase
• NCBI Official Symbol: sod4
• NCBI Official Synonym Symbols: sod4ap; SODCC.2; GRMZM2G169890
• NCBI Protein Information: superoxide dismutase [Cu-Zn] 4AP
• UniProt Protein Name: Superoxide dismutase [Cu-Zn] 4AP
• Protein Family: Cell surface superoxide dismutase
• UniProt Gene Name: SODCC.2
• UniProt Synonym Gene Names: SOD4AP

Uniprot Description

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.