Typical Testing Data/Standard Curve (for reference only)
Bovine Follitropin subunit beta (FSHB) ELISA Kit | FSHB elisa kit
Bovine Follitropin subunit beta (FSHB) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: FSHbeta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FSHbeta antibody and an FSHbeta-HRP conjugate. The assay sample and buffer are incubated together with FSHbeta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FSHbeta concentration since FSHbeta from samples and FSHbeta-HRP conjugate compete for the anti-FSHbeta antibody binding site. Since the number of sites is limited, as more sites are occupied by FSHbeta from the sample, fewer sites are left to bind FSHbeta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FSHbeta concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The pituitary glycoprotein hormone family includes follicle-stimulating hormone, luteinizing hormone, chorionic gonadotropin, and thyroid-stimulating hormone. All of these glycoproteins consist of an identical alpha subunit and a hormone-specific beta subunit. This gene encodes the beta subunit of follicle-stimulating hormone. In conjunction with luteinizing hormone, follicle-stimulating hormone induces egg and sperm production. Alternative splicing results in two transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
FSHB: Stimulates development of follicle and spermatogenesis in the reproductive organs. Defects in FSHB are a cause of isolated follicle- stimulating hormone deficiency (IFSHD). Selective follicle-stimulating hormone deficiency is an uncommon cause of infertility, producing amenorrhea and hypogonadism in women and oligo or azoospermia with normal testosterone levels in normally virilised men. Belongs to the glycoprotein hormones subunit beta family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: cytoplasm; extracellular region
Molecular Function: follicle-stimulating hormone activity; protein binding; hormone activity
Biological Process: progesterone biosynthetic process; follicle-stimulating hormone signaling pathway; peptide hormone processing; female gamete generation; Sertoli cell proliferation; female pregnancy; signal transduction; cellular protein metabolic process; regulation of osteoclast differentiation; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of bone resorption; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell migration
Disease: Follicle-stimulating Hormone Deficiency, Isolated
Research Articles on FSHB
Similar Products
Product Notes
The Bovine FSHB fshb (Catalog #AAA7253353) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7253353 ELISA Kit recognizes Bovine FSHB. It is sometimes possible for the material contained within the vial of "Follitropin subunit beta (FSHB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
