Rabbit anti-Human LTK Polyclonal Antibody | anti-LTK antibody

LTK, NT (Leukocyte Receptor Tyrosine Kinase, Leukocyte Tyrosine Kinase Receptor, Protein Tyrosine Kinase 1, TYK1) (Biotin)

Cat. Num. AAA6485519

• Gene Names: LTK; TYK1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: LTK; Polyclonal Antibody; NT (Leukocyte Receptor Tyrosine Kinase; Leukocyte Tyrosine Kinase Receptor; Protein Tyrosine Kinase 1; TYK1) (Biotin); EC=2.7.10.1; anti-LTK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LTK.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-LTK antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 42-72 in the N-terminal region of human LTK, Swiss/UniProt Accession: P29376.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot to detect LTK in placenta cell lysate usingMBS627671.)

Western Blot (WB)

(Western Blot human skeletal muscle lysate (20ug) using MBS627671 (1:2000). Secondary IgG (HRP) Goat Anti-Rabbit at 1:10,000. Predicted band size: 92kD. Blocking/Dilution buffer: 5%. NFDM/TBST.)

Related Product Information for anti-LTK antibody

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

Product Categories/Family for anti-LTK antibody

Antibodies; Receptors; Tyrosine Kinase

References

1. Toyoshima, H., et al., Proc. Natl. Acad. Sci. USA 90(12):5404-5408 (1993). 2. Krolewski, J.J., et al., EMBO J. 10(10):2911-2919 (1991). 3. Maru, Y., et al., Oncogene Res. 5(3):199-204 (1990).

NCBI and Uniprot Product Information

• NCBI GI #: 208879423
• NCBI GeneID: 4058
• NCBI Accession #: NP_001129157.1
• NCBI GenBank Nucleotide #: NM_001135685.1
• UniProt Accession #: P29376; A6NNJ8; B4DL89; E9PFX4
• Molecular Weight: 91,681 Da
• NCBI Official Full Name: leukocyte tyrosine kinase receptor isoform 3
• NCBI Official Synonym Full Names: leukocyte receptor tyrosine kinase
• NCBI Official Symbol: LTK
• NCBI Official Synonym Symbols: TYK1
• NCBI Protein Information: leukocyte tyrosine kinase receptor; protein tyrosine kinase 1
• UniProt Protein Name: Leukocyte tyrosine kinase receptor
• Protein Family: Leukocyte tyrosine kinase receptor
• UniProt Gene Name: LTK
• UniProt Synonym Gene Names: TYK1
• UniProt Entry Name: LTK_HUMAN

NCBI Description

The protein encoded by this gene is a member of the ros/insulin receptor family of tyrosine kinases. Tyrosine-specific phosphorylation of proteins is a key to the control of diverse pathways leading to cell growth and differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

LTK: Orphan receptor with a tyrosine-protein kinase activity. The exact function of this protein is not known. Studies with chimeric proteins (replacing its extracellular region with that of several known growth factor receptors, such as EGFR and CSFIR) demonstrate its ability to promote growth and specifically neurite outgrowth, and cell survival. Signaling appears to involve the PI3 kinase pathway. Involved in regulation of the secretory pathway involving endoplasmic reticulum (ER) export sites (ERESs) and ER to Golgi transport. Homodimer when bound to ligand (Probable). Part a complex including LTK, TNK2 and GRB2, in which GRB2 promotes LTK recruitment by TNK2. Expressed in non-hematopoietic cell lines and T- and B-cell lines. Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.1; TK group; Alk family

Chromosomal Location of Human Ortholog: 15q15.1-q21.1

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; ATP binding; protein kinase activity

Biological Process: cell proliferation; phosphoinositide 3-kinase cascade; peptidyl-tyrosine phosphorylation; signal transduction; protein amino acid phosphorylation; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis