Rabbit anti-Human PSMD13 Polyclonal Antibody | anti-PSMD13 antibody

Anti-PSMD13 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104029

• Gene Names: PSMD13; S11; Rpn9; p40.5; HSPC027
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: PSMD13; Polyclonal Antibody; Anti-PSMD13 Antibody; Rabbit Polyclonal; PSMD13 Antibody; Rabbit PAb; Antigen Affinity Purified; proteasome (prosome; macropain) 26S subunit; non-ATPase; 13; Anti-HSPC027 Antibody; Anti-p40.5 Antibody; Anti-Rpn9 Antibody; Anti-S11 Antibody; anti-PSMD13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PSMD13
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PSMD13 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the center region of the Human PSMD13
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PSMD13, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PSMD13 rabbit polyclonal antibody at 1:500 dilution Lane A: 293T Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:45 kDa)

Related Product Information for anti-PSMD13 antibody

PSMD13 (Proteasome 26S Subunit, Non-ATPase 13) is a Protein Coding gene. The PSMD13 gene, located on 11p15.5, is conserved in chimpanzee, dog, cow, mouse, rat, and chicken, etc. This gene encodes a non-ATPase subunit of the 19S regulator. PSMD13, a newly identified negative regulator of RPE65, plays a critical role in regulating the pathogenicity of three mutations (L22P, T101I, and L408P) by mediating rapid degradation of mutated RPE65s via ubiquitination-and proteasome-dependent non-lysosomal pathway. Two transcripts encoding different isoforms have been described. PSMD13 is widely expressed in the appendix, lymph node, and other tissues. The related pathways of PSMD13 are Regulation of activated PAK-2p34 by proteasome-mediated degradation and Antigen processing-Cross presentation.

References

Li S, et al. (2015) Temperature-sensitive retinoid isomerase activity of rpe65 mutants associated with leber congenital amaurosis. J Biochem 158 (2): 115-125.Li S, et al. (2014) Rescue of enzymatic function for disease-associated rpe65 proteins containing various missense mutations in non-active sites. J Biol Chem 289 (27): 18943-18956.Bellizzi D, et al. (2007) Characterization of a bidirectional promoter shared between two human genes related to aging: Sirt3 and psmd13. Genomics 89 (1): 143-150.

NCBI and Uniprot Product Information

• NCBI GI #: 157502193
• NCBI GeneID: 5719
• NCBI Accession #: NP_002808
• NCBI GenBank Nucleotide #: NM_002817.3
• UniProt Accession #: Q9UNM6; O75831; Q53XU2; Q9UNV3; B3KT15
• Molecular Weight: 45.3kDa (399aa)
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 13 isoform 1
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 13
• NCBI Official Symbol: PSMD13
• NCBI Official Synonym Symbols: S11; Rpn9; p40.5; HSPC027
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 13
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 13
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD13

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair.