Rabbit anti-Human PSMC6 Polyclonal Antibody | anti-PSMC6 antibody

PSMC6 antibody

Cat. Num. AAA9403561

• Gene Names: PSMC6; P44; p42; SUG2; CADP44; HEL-S-73
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: PSMC6; Polyclonal Antibody; PSMC6 antibody; anti-PSMC6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Supplied in 0.1M Tris-buffered saline with 10% Glycerol (pH7.0). 0.01% Thimerosal was added as a preservative.
• Sequence Length: 403

• Applicable Applications for anti-PSMC6 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500-1:3000; Immunohistochemistry: 1:100-1:250; Immunofluorescence: 1:100-1:200
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 285 of Human PSMC6
• Target Name: PSMC6
• Preparation and Storage: Store at -20 degree C for long term preservation (recommended). Store at 4 degree C for short term use.

Testing Data

(Sample (30 ug of whole cell lysate)A: 293T 7.5% SDS PAGEPrimary antibody diluted at 1: 500)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded A549 xenograft, using PSMC6 antibody at 1: 100 dilution.)

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PSMC6 antibody at 1: 500 dilution.)

Related Product Information for anti-PSMC6 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq]

Product Categories/Family for anti-PSMC6 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 195539395
• NCBI GeneID: 5706
• NCBI Accession #: NP_002797
• NCBI GenBank Nucleotide #: NM_002806.3
• Molecular Weight: 44,173 Da
• NCBI Official Full Name: 26S protease regulatory subunit 10B
• NCBI Official Synonym Full Names: proteasome 26S subunit, ATPase 6
• NCBI Official Symbol: PSMC6
• NCBI Official Synonym Symbols: P44; p42; SUG2; CADP44; HEL-S-73
• NCBI Protein Information: 26S protease regulatory subunit 10B
• UniProt Protein Name: 26S protease regulatory subunit 10B
• Protein Family: 26S protease regulatory
• UniProt Gene Name: PSMC6
• UniProt Synonym Gene Names: SUG2
• UniProt Entry Name: PRS10_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. Pseudogenes have been identified on chromosomes 8 and 12. [provided by RefSeq, Jul 2008]

Uniprot Description

RPT4: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. Belongs to the AAA ATPase family.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 14q22.1

Cellular Component: proteasome complex; nucleoplasm; membrane; nucleus; cytosol

Molecular Function: protein binding, bridging; protein binding; ATPase activity; ATP binding

Biological Process: ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis