Western Blot (WB)
(PSMB8 rabbit polyclonal antibody. Western Blot analysis of PSMB8 expression in human kidney.)
Rabbit anti-Human, Mouse PSMB8 Polyclonal Antibody | anti-PSMB8 antibody
PSMB8 (LMP7, PSMB5i, RING10, Y2, Proteasome Subunit beta Type-8, Low Molecular Mass Protein 7, Macropain Subunit C13, Multicatalytic Endopeptidase Complex Subunit C13, Proteasome Component C13, Proteasome Subunit beta-5i, Really Interesting New Gene 10 Pr
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Western Blot (WB)
(PSMB8 rabbit polyclonal antibody. Western Blot analysis of PSMB8 expression in human kidney.)
Western Blot (WB)
(PSMB8 rabbit polyclonal antibody. Western Blot analysis of PSMB8 expression in human kidney.)
Western Blot (WB)
(PSMB8 rabbit polyclonal antibody. Western Blot analysis of PSMB8 expression in mouse liver.)
Western Blot (WB)
(PSMB8 rabbit polyclonal antibody. Western Blot analysis of PSMB8 expression in mouse liver.)
Western Blot (WB)
(Western Blot analysis of PSMB8 expression in transfected 293T cell line by PSMB8 polyclonal antibody. Lane 1: PSMB8 transfected lysate (29.8kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of PSMB8 expression in transfected 293T cell line by PSMB8 polyclonal antibody. Lane 1: PSMB8 transfected lysate (29.8kD). Lane 2: Non-transfected lysate.)
NCBI and Uniprot Product Information
NCBI Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes. Ref.12 Ref.21 Ref.25 Ref.29
Catalytic activity: Cleavage of peptide bonds with very broad specificity.
Subunit structure: The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. This subunit is part of the immunoproteasome where it displaces the equivalent housekeeping subunit PSMB5. Component of the spermatoproteasome, a form of the proteasome specifically found in testis. Directly interacts with POMP. Interacts with HIV-1 TAT protein. Interacts with TAP1. Ref.16 Ref.19 Ref.20
Subcellular location: Cytoplasm
By similarity. Nucleus
By similarity.
Developmental stage: Highly expressed in immature dendritic cells (at protein level). Ref.15
Induction: Up-regulated by IFNG/IFN-gamma and IRF1 (at protein level). Up-regulated by TNF (at protein level). Up-regulated by tetrodotoxin (TTX) in glial cells. Up-regulated in Crohn's bowel disease (CD). Down-regulated by the selective inhibitor PR-957. Down-regulated in mature dendritic cells by HSV-1 infection. Up-regulated by heat shock treatment. Ref.13 Ref.14 Ref.17 Ref.18 Ref.22 Ref.23 Ref.25 Ref.26 Ref.27
Post-translational modification: Autocleaved. The resulting N-terminal Thr residue of the mature subunit is responsible for the nucleophile proteolytic activity
By similarity.
Involvement in disease: Nakajo syndrome (NKJO) [MIM:256040]: An autosomal recessive autoinflammatory disorder characterized by early childhood onset of recurrent fever, joint stiffness and severe contractures of the hands and feet, and erythematous skin lesions with subsequent development of lipodystrophy and laboratory evidence of immune dysregulation. Accompanying features may include muscle weakness and atrophy, hepatosplenomegaly, and microcytic anemia.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.29 Ref.30 Ref.31 Ref.32Mutation Met-75 has been found in chronic atypical neutrophilic dermatosis with lipodystrophy and elevated temperature syndrome (CANDLE syndrome). CANDLE patients have some overlapping features with NKJO patients, including a cutaneous eruption and lipodystrophy. They show a characteristic neutrophilic dermatosis with a mononuclear interstitial infiltrate in the dermis that seems pathognomonic for CANDLE syndrome (Ref.31).
Sequence similarities: Belongs to the peptidase T1B family.
Research Articles on PSMB8
Similar Products
Product Notes
The PSMB8 psmb8 (Catalog #AAA649824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSMB8 (LMP7, PSMB5i, RING10, Y2, Proteasome Subunit beta Type-8, Low Molecular Mass Protein 7, Macropain Subunit C13, Multicatalytic Endopeptidase Complex Subunit C13, Proteasome Component C13, Proteasome Subunit beta-5i, Really Interesting New Gene 10 Pr reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PSMB8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the PSMB8 psmb8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MLIGTPTPRD TTPSSWLTSS LLVEAAPLDD TTLPTPVSSG CPGLEPTEFF QSLGGDGERN VQIEMAHGTT TLAFKFQHGV IAAVDSRASA GSYISALRVN KVIEINPYLL GTMSGCAADC QYWERLLAKE CRLYYLRNGE RISVSAASKL LSNMMCQYRG MGLSMGSMIC GWDKKGPGLY YVDEHGTRLS GNMFSTGSGN TYAYGVMDSG YRPNLSPEEA YDLGRRAIAY ATHRDSYSGG VVNMYHMKED GWVKVESTDV SDLLHQYREA NQ. It is sometimes possible for the material contained within the vial of "PSMB8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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