Testing Data
(Binding of C2425-01QX to CD27+ human PBMC.)
hCD70-muCD8 Fusion Protein
hCD70-muCD8 Fusion Protein (human CD70-murine CD8 Fusion Protein)
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
hCD70-muCD8 Fusion Protein; hCD70-muCD8 Fusion Protein (human CD70-murine CD8 Fusion Protein); hCD70-muCD8 Fusion Protein protein
Host
CHO cells
Specificity
Binds to cell surface CD27 on a subset of T cells.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
Applicable Applications for hCD70-muCD8 Fusion Protein protein
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in ELISA and Flow Cytometry.
Dilution: Flow Cytometry: 10ug/ml; 80ul labels 5x10e5 ficoll prepared human peripheral blood mononuclear cells.
Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated for 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding).
2. They were incubated for 45 minutes on ice with 80ul of C2425-01QX at 10ug/ml. 3. Cells were washed twice and incubated with C2259-11D, after which they were washed three times, fixed and analyzed by FACS.
4. A net 57% sub population of the cells stained positive with a mean shift of 1.15 log10 fluorescent units when compared to a buffer control. Binding was 99% blocked when reagent was first pre incubated with an excess of CD-70 antibody.
Dilution: Flow Cytometry: 10ug/ml; 80ul labels 5x10e5 ficoll prepared human peripheral blood mononuclear cells.
Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated for 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding).
2. They were incubated for 45 minutes on ice with 80ul of C2425-01QX at 10ug/ml. 3. Cells were washed twice and incubated with C2259-11D, after which they were washed three times, fixed and analyzed by FACS.
4. A net 57% sub population of the cells stained positive with a mean shift of 1.15 log10 fluorescent units when compared to a buffer control. Binding was 99% blocked when reagent was first pre incubated with an excess of CD-70 antibody.
Molecular Structure
A soluble molecule consisting of the extracellular domain (167aa) of murine CD8 alpha fused to 146 (c-term) amino acids of the extracellular domain of human CD70, with a predicted monomeric weight of 34.8kD. Extracellular mature murine CD8alpha: (28)KPQAPEL....GLDFACD(194); Linking amino acids: GT; extracellular human CD70: (48)LPLESL....VQWVRP(193).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Testing Data
(Binding of C2425-01QX to CD27+ human PBMC.)
Testing Data
(Binding of C2425-01QX to CD27+ human PBMC.)
Related Product Information for hCD70-muCD8 Fusion Protein protein
Human CD70 (TNFSF7) is the ligand for CD27 (TNFRSF7) and is notably absent on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. Recombinant CD70-muCD8 binds to cell surface CD27 on human peripheral blood leukocytes.
Product Categories/Family for hCD70-muCD8 Fusion Protein protein
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Product Notes
The hCD70-muCD8 Fusion Protein (Catalog #AAA634480) is a Protein produced from CHO cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's hCD70-muCD8 Fusion Protein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Flow Cytometry (FC/FACS). Suitable for use in ELISA and Flow Cytometry. Dilution: Flow Cytometry: 10ug/ml; 80ul labels 5x10e5 ficoll prepared human peripheral blood mononuclear cells. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated for 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding). 2. They were incubated for 45 minutes on ice with 80ul of C2425-01QX at 10ug/ml. 3. Cells were washed twice and incubated with C2259-11D, after which they were washed three times, fixed and analyzed by FACS. 4. A net 57% sub population of the cells stained positive with a mean shift of 1.15 log10 fluorescent units when compared to a buffer control. Binding was 99% blocked when reagent was first pre incubated with an excess of CD-70 antibody. Researchers should empirically determine the suitability of the hCD70-muCD8 Fusion Protein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "hCD70-muCD8 Fusion Protein, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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