Rabbit PSMB10 Polyclonal Antibody | anti-PSMB10 antibody

PSMB10 antibody

Cat. Num. AAA9414616

• Gene Names: PSMB10; LMP10; MECL1; beta2i
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: PSMB10; Polyclonal Antibody; PSMB10 antibody; LMP10; MECL1; beta2i; anti-PSMB10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PSMB10 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 273

• Applicable Applications for anti-PSMB10 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:100
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human PSMB10.
• Target Name: PSMB10
• Preparation and Storage: Store at -20 degree C

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using PSMB10 antibody. Blue: DAPI for nuclear staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody at dilution of 1:200 (400x lens).)

Related Product Information for anti-PSMB10 antibody

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.

Product Categories/Family for anti-PSMB10 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4506191
• NCBI GeneID: 5699
• NCBI Accession #: NP_002792.1
• NCBI GenBank Nucleotide #: NM_002801.3
• UniProt Accession #: P40306; Q5U098; B2R5J4
• Molecular Weight: SDS-PAGE MW: 29kd
• NCBI Official Full Name: proteasome subunit beta type-10
• NCBI Official Synonym Full Names: proteasome subunit beta 10
• NCBI Official Symbol: PSMB10
• NCBI Official Synonym Symbols: LMP10; MECL1; beta2i
• NCBI Protein Information: proteasome subunit beta type-10
• UniProt Protein Name: Proteasome subunit beta type-10
• Protein Family: Proteasome
• UniProt Gene Name: PSMB10
• UniProt Synonym Gene Names: LMP10; MECL1
• UniProt Entry Name: PSB10_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMB10: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Belongs to the peptidase T1B family.

Protein type: Proteasome complex; EC 3.4.25.1; Protease

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytosol

Molecular Function: threonine endopeptidase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; cell morphogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; humoral immune response; T cell proliferation; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis