Rabbit PSMA5 Polyclonal Antibody | anti-PSMA5 antibody

PSMA5 (Proteasome Alpha 5, PSC5, ZETA) (HRP)

Cat. Num. AAA6433529

• Gene Names: PSMA5; PSC5; ZETA
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: PSMA5; Polyclonal Antibody; PSMA5 (Proteasome Alpha 5; PSC5; ZETA) (HRP); anti-PSMA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PSMA5. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PSMA5 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human PSMA5.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PSMA5 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using PSMA5 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-PSMA5 antibody

Antibodies; Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 315138981
• NCBI GeneID: 5686
• NCBI Accession #: NP_001186701.1
• NCBI GenBank Nucleotide #: NM_001199772.1
• UniProt Accession #: P28066; Q3T1C1; Q6IBF7; B2R8F6; B4E2V4
• Molecular Weight: 20,035 Da
• NCBI Official Full Name: proteasome subunit alpha type-5 isoform 2
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, alpha type, 5
• NCBI Official Symbol: PSMA5
• NCBI Official Synonym Symbols: PSC5; ZETA
• NCBI Protein Information: proteasome subunit alpha type-5; macropain subunit zeta; macropain zeta chain; multicatalytic endopeptidase complex zeta chain; proteasome alpha 5 subunit; proteasome component 5; proteasome subunit zeta; proteasome zeta chain
• UniProt Protein Name: Proteasome subunit alpha type-5
• UniProt Gene Name: PSMA5
• UniProt Entry Name: PSA5_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

PSMA5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA5 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Up-regulated in colon cancer cell lines. Up-regulated in fetal Down syndrome (DS) brain. May be the target of the transcriptional activator NFE2L2. Expressed in fetal brain. Belongs to the peptidase T1A family.

Protein type: Protease; Proteasome complex; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytoplasm; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle