Rabbit PSMA3 Polyclonal Antibody | anti-PSMA3 antibody

PSMA3 Conjugated Antibody

Cat. Num. AAA9443397

• Gene Names: PSMA3; HC8; PSC3
• Reactivity: Human, Mouse, Rat
• Applications: Most Applications
• Synonyms: PSMA3; Polyclonal Antibody; PSMA3 Conjugated Antibody; HC8; MGC12306; MGC32631; PSC3; anti-PSMA3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total PSMA3 protein.
• Form/Format: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6, 5mg/ml Bovine Serum Albumin, 0.02% Sodium Azide

• Applicable Applications for anti-PSMA3 antibody: Most Applications
• Application Notes: Suggested Dilution:; AF350 conjugated: most applications: 1:50-1:250; AF405 conjugated: most applications: 1:50-1:250; AF488 conjugated: most applications: 1:50-1:250; AF555 conjugated: most applications: 1:50-1:250; AF594 conjugated: most applications: 1:50-1:250; AF647 conjugated: most applications: 1:50-1:250; AF680 conjugated: most applications: 1:50-1:250; AF750 conjugated: most applications: 1:50-1:250; Biotin conjugated: working with enzyme-conjugated streptavidin, most applications: 1:50-1:1000
• Immunogen: Recombinant protein of human PSMA3.
• Conjugation: Biotin AF350 AF405 AF488 AF555 AF594 AF647 AF680 AF750
• Excitation Emission: AF350: 346nm/442nm
• Preparation and Storage: Store at 4 degree C for 6 months.

Related Product Information for anti-PSMA3 antibody

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of alpha subunits (called PSMAs), and the two inner layers are identical rings composed of beta subunits. While the catalytic sites are located on the beta rings (1-3), the alpha subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different alpha and ten different beta proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades poly-ubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9).

NCBI and Uniprot Product Information

• NCBI GI #: 4506183
• NCBI GeneID: 5684
• NCBI Accession #: NP_002779.1
• NCBI GenBank Nucleotide #: NM_002788.3
• UniProt Accession #: P25788; Q86U83; Q8N1D8; Q9BS70; B2RCK6
• Molecular Weight: 28,433 Da
• NCBI Official Full Name: proteasome subunit alpha type-3 isoform 1
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, alpha type, 3
• NCBI Official Symbol: PSMA3
• NCBI Official Synonym Symbols: HC8; PSC3
• NCBI Protein Information: proteasome subunit alpha type-3; macropain subunit C8; proteasome subunit C8; proteasome component C8; multicatalytic endopeptidase complex subunit C8
• UniProt Protein Name: Proteasome subunit alpha type-3
• Protein Family: Phenol-soluble modulin alpha 3 peptide
• UniProt Gene Name: PSMA3
• UniProt Synonym Gene Names: HC8; PSC8
• UniProt Entry Name: PSA3_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMA3: a proteasomal protein of the T1A peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Two alternatively spliced isoforms have been identified.

Protein type: EC 3.4.25.1; Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 14q23

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; cytoplasm; cytosol; nucleus

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis